Effects of neutrophil depletion in the local pathological alterations and muscle regeneration. in mice injected with Bothrops jararaca snake venom

dc.creatorTeixeira, CFP
dc.creatorChaves, F
dc.creatorZamuner, SR
dc.creatorFernandes, CM
dc.creatorZuliani, JP
dc.creatorCruz-Hofling, MA
dc.creatorFernandes, I
dc.creatorGutierrez, JM
dc.date2005
dc.dateAPR
dc.date2014-11-17T21:13:13Z
dc.date2015-11-26T16:46:40Z
dc.date2014-11-17T21:13:13Z
dc.date2015-11-26T16:46:40Z
dc.date.accessioned2018-03-28T23:32:34Z
dc.date.available2018-03-28T23:32:34Z
dc.identifierInternational Journal Of Experimental Pathology. Blackwell Publishing Ltd, v. 86, n. 2, n. 107, n. 115, 2005.
dc.identifier0959-9673
dc.identifierWOS:000228486900005
dc.identifier10.1111/j.0959-9673.2005.00419.x
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/65029
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/65029
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/65029
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1274598
dc.descriptionIn order to study the role of neutrophils in the acute local pathological alterations induced by Bothrops jararaca snake venom, and in the process of skeletal muscle regeneration that follows, an experimental model was developed in mice pretreated with either an antimouse granulocyte rat monoclonal immunoglobulin G, which induces a profound neutropenia, or an isotype-matched control antibody. B. jararaca venom induced prominent haemorrhage and oedema, but only a moderate myonecrosis. No significant differences were observed in the extent of local haemorrhage, oedema and myonecrosis between neutropenic and control mice, suggesting that neutrophils do not play a determinant role in the acute pathological alterations induced by B. jararaca venom in this experimental model. Moreover, no differences were observed in skeletal muscle regeneration between these two experimental groups. In both the cases, limited areas of myonecrosis were associated with a drastic damage to the microvasculature and a scarce inflammatory infiltrate, with the consequent lack of removal of necrotic debris during the first week, resulting in a poor regenerative response at this time interval. Subsequently, a similar regenerative process occurred in both groups, and by 30 days, necrotic areas were substituted by groups of small regenerating muscle fibres. It is suggested that the drastic effect exerted by B. jararaca venom in the microvasculature precludes an effective access of inflammatory cells to necrotic areas, thereby compromising an effective removal of necrotic debris; this explains the poor regenerative response observed during the first week and the fact that there were no differences between neutropenic and control mice. As neutropenia. in this model lasted only 7 days, the successful regenerative process observed at 30 days is associated with revascularization of necrotic regions and with a successful removal by phagocytes of necrotic debris in both groups.
dc.description86
dc.description2
dc.description107
dc.description115
dc.languageen
dc.publisherBlackwell Publishing Ltd
dc.publisherOxford
dc.publisherInglaterra
dc.relationInternational Journal Of Experimental Pathology
dc.relationInt. J. Exp. Pathol.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjecthaemorrhage
dc.subjectmuscle regeneration
dc.subjectmyonecrosis
dc.subjectneutrophils
dc.subjectsnake venom
dc.subjectAsper Terciopelo
dc.subjectCrude Venom
dc.subjectHemorrhage
dc.subjectEdema
dc.subjectMetalloproteinase
dc.subjectPurification
dc.subjectMyonecrosis
dc.subjectRat
dc.subjectJararhagin
dc.subjectProteins
dc.titleEffects of neutrophil depletion in the local pathological alterations and muscle regeneration. in mice injected with Bothrops jararaca snake venom
dc.typeArtículos de revistas


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