Schistosoma mansoni: Evaluation of an RNAi-based treatment targeting HGPRTase gene

dc.creatorPereira, TC
dc.creatorPascoal, VDB
dc.creatorMarchesini, RB
dc.creatorMaia, IG
dc.creatorMagalhaes, LA
dc.creatorZanotti-Magalhaes, EM
dc.creatorLopes-Cendes, I
dc.date2008
dc.dateAPR
dc.date2014-11-17T21:11:37Z
dc.date2015-11-26T16:46:38Z
dc.date2014-11-17T21:11:37Z
dc.date2015-11-26T16:46:38Z
dc.date.accessioned2018-03-28T23:32:32Z
dc.date.available2018-03-28T23:32:32Z
dc.identifierExperimental Parasitology. Academic Press Inc Elsevier Science, v. 118, n. 4, n. 619, n. 623, 2008.
dc.identifier0014-4894
dc.identifierWOS:000254885000026
dc.identifier10.1016/j.exppara.2007.11.017
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/71508
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/71508
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/71508
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1274588
dc.descriptionHypoxanthine-guanine phosphoribosyltransferase (HGPRTase) is an essential gene of the parasite Schistosoma mansoni and it is well conserved in its hosts (mouse and human) at the protein but not at the RNA level. This feature prompted us to assess RNA interference (RNAi) to combat schistosomiasis. Small interfering RNAs (siRNAs) were Produced against HGPRTase, injected in infected mice and the number of worms was counted six days after injection. The total number of parasites was reduced by approximately 27% after treatment. RT-PCR analyzes showed a significant reduction in parasite target mRNA but not in host's homologue. The use of low doses of molecules did not oversaturate si- or miRNA pathways as mice survival rates were not affected by siRNAs. This is the first successful in vivo demonstration of a RNAi-based treatment against schistosomiasis. We believe that improvements in molecule delivery and an increase on siRNA dose could rapidly eliminate parasite. (c) 2007 Elsevier Inc. All rights reserved.
dc.description118
dc.description4
dc.description619
dc.description623
dc.languageen
dc.publisherAcademic Press Inc Elsevier Science
dc.publisherSan Diego
dc.publisherEUA
dc.relationExperimental Parasitology
dc.relationExp. Parasitol.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectSchistosoma mansoni
dc.subjectTrematode
dc.subjecthypoxanthine-guanine
dc.subjectphosphoribosyltransferase
dc.subjectPlasmid Dna
dc.subjectIn-vivo
dc.subjectInterference
dc.subjectExpression
dc.subjectMice
dc.subjectCells
dc.subjectSirna
dc.titleSchistosoma mansoni: Evaluation of an RNAi-based treatment targeting HGPRTase gene
dc.typeArtículos de revistas


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