Fragmentation features of intermolecular cross-linked peptides using N-hydroxy-succinimide esters by MALDI- and ESI-MS/MS for use in structural proteomics

dc.creatorSantos, LFA
dc.creatorIglesias, AH
dc.creatorGozzo, FC
dc.date2011
dc.dateAUG
dc.date2014-07-30T17:37:03Z
dc.date2015-11-26T16:46:27Z
dc.date2014-07-30T17:37:03Z
dc.date2015-11-26T16:46:27Z
dc.date.accessioned2018-03-28T23:32:19Z
dc.date.available2018-03-28T23:32:19Z
dc.identifierJournal Of Mass Spectrometry. Wiley-blackwell, v. 46, n. 8, n. 742, n. 750, 2011.
dc.identifier1076-5174
dc.identifierWOS:000294521800003
dc.identifier10.1002/jms.1951
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/67131
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/67131
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1274533
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionThe use of mass spectrometry coupled with chemical cross-linking of proteins has become one of the most useful tools for proteins structure and interactions studies. One of the challenges in these studies is the identification of the cross-linked peptides. The interpretation of the MS/MS data generated in cross-linking experiments using N-hydroxy succinimide esters is not trivial once a new amide bond is formed allowing new fragmentation pathways, unlike linear peptides. Intermolecular cross-linked peptides occur when two different peptides are connected by the cross-linker and they yield information on the spatial proximity of different domains (within a protein) or proteins (within a complex). In this article, we report a detailed fragmentation study of intermolecular cross-linked peptides, generated from a set of synthetic peptides, using both ESI and MAID! to generate the precursor ions. The fragmentation features observed here can be helpful in the interpretation and identification of cross-linked peptides present in cross-linking experiments and be further implemented in search engine's algorithms. Copyright (C) 2011 John Wiley & Sons, Ltd.
dc.description46
dc.description8
dc.description742
dc.description750
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFAPESP [FAPESP 2004/14846-0, FINEP 01.07.0290.00]
dc.descriptionFAPESP [CNPq 573672/2008-3, FAPESP 08/57805-2]
dc.descriptionFAPESP [2007/55930-1]
dc.languageen
dc.publisherWiley-blackwell
dc.publisherMalden
dc.publisherEUA
dc.relationJournal Of Mass Spectrometry
dc.relationJ. Mass Spectrom.
dc.rightsfechado
dc.rightshttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.sourceWeb of Science
dc.subjectcross-linking
dc.subjectpeptide fragmentation
dc.subjectmass spectrometry
dc.subjectprotein structure
dc.subjectMALDI
dc.subjectESI
dc.subjectProtein-protein Interactions
dc.subjectSelective Chemical-modification
dc.subjectTransform Mass-spectrometry
dc.subjectTop-down Approach
dc.subjectConformational-changes
dc.subjectInduced Dissociation
dc.subjectSurface-topology
dc.subjectLinking Analysis
dc.subjectIdentification
dc.subjectComplexes
dc.titleFragmentation features of intermolecular cross-linked peptides using N-hydroxy-succinimide esters by MALDI- and ESI-MS/MS for use in structural proteomics
dc.typeArtículos de revistas


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