O-2 consumption rates along the growth curve: new insights into Trypanosoma cruzi mitochondrial respiratory chain

dc.creatorSilva, TM
dc.creatorPeloso, EF
dc.creatorVitor, SC
dc.creatorRibeiro, LHG
dc.creatorGadelha, FR
dc.date2011
dc.dateAUG
dc.date2014-07-30T14:48:59Z
dc.date2015-11-26T16:46:11Z
dc.date2014-07-30T14:48:59Z
dc.date2015-11-26T16:46:11Z
dc.date.accessioned2018-03-28T23:31:56Z
dc.date.available2018-03-28T23:31:56Z
dc.identifierJournal Of Bioenergetics And Biomembranes. Springer/plenum Publishers, v. 43, n. 4, n. 409, n. 417, 2011.
dc.identifier0145-479X
dc.identifierWOS:000298009700008
dc.identifier10.1007/s10863-011-9369-0
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/62282
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/62282
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1274452
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionUnderstanding the energy-transduction pathways employed by Trypanosoma cruzi, the etiological agent of Chagas disease, may lead to the identification of new targets for development of a more effective therapy. Herein, the contribution of different substrates for O-2 consumption rates along T. cruzi epimastigotes (Tulahuen 2 and Y strains) growth curve was evaluated. O-2 consumption rates were higher at the late stationary phase not due to an increase on succinate-dehydrogenase activity. Antimycin A and cyanide did not totally inhibit the mitochondrial respiratory chain (MRC). Malonate at 10 or 25 mM was not a potent inhibitor of complex II. Comparing complex II and III, the former appears to be the primary site of H2O2 release. An update on T. cruzi MRC is presented that together with our results bring important data towards the understanding of the parasite's MRC. The findings mainly at the stationary phase could be relevant for epimastigotes transformation into the metacyclic form, and in this sense deserves further attention.
dc.description43
dc.description4
dc.description409
dc.description417
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.languageen
dc.publisherSpringer/plenum Publishers
dc.publisherNew York
dc.publisherEUA
dc.relationJournal Of Bioenergetics And Biomembranes
dc.relationJ. Bioenerg. Biomembr.
dc.rightsfechado
dc.rightshttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.sourceWeb of Science
dc.subjectTrypanosoma cruzi
dc.subjectMitochondrial respiratory chain
dc.subjectOxygen consumption
dc.subjectReactive oxygen species
dc.subjectLeishmania-donovani Promastigotes
dc.subjectEnergy-metabolism
dc.subjectOxidative Stress
dc.subjectElectron-transport
dc.subjectGenome Project
dc.subjectChagas-disease
dc.subjectCell-death
dc.subjectComplex-i
dc.subjectCl Brener
dc.subject2 Strains
dc.titleO-2 consumption rates along the growth curve: new insights into Trypanosoma cruzi mitochondrial respiratory chain
dc.typeArtículos de revistas


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