Monitoring of florfenicol residues in fish muscle by HPLC-UV with confirmation of suspect results by LC-MS/MS

dc.creatorGranja, RHMM
dc.creatorde Lima, AC
dc.creatorPatel, RK
dc.creatorSalerno, AG
dc.creatorWanschel, ACBA
dc.date2012
dc.dateAUG
dc.date2014-07-30T14:03:51Z
dc.date2015-11-26T16:45:56Z
dc.date2014-07-30T14:03:51Z
dc.date2015-11-26T16:45:56Z
dc.date.accessioned2018-03-28T23:31:38Z
dc.date.available2018-03-28T23:31:38Z
dc.identifierDrug Testing And Analysis. Wiley-blackwell, v. 4, n. 125, n. 129, 2012.
dc.identifier1942-7603
dc.identifierWOS:000306957000016
dc.identifier10.1002/dta.1362
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/57847
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/57847
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1274371
dc.descriptionFlorfenicol, a derivative of thiamphenicol in which the hydroxyl group at C-3 has been replaced with fluorine, is listed by the World Health Organization as an antibacterial agent for human medicine that is critically important in risk management strategies for non-human use. AOAC International has also identified it as an important molecule for the development of effective methods for the seafood sector. Following inspection missions from the European Union and United States of America, it was introduced in the Brazilian Residues Control Program to fulfill export and internal national requirements with a Maximum Residue Limit of 800?mu g/kg. A high performance liquid chromatography method with ultraviolet detection at a wavelength of 230?nm (??=?230?nm) for the detection of florfenicol in fish muscle was developed and validated according to the Brazilian Regulation 24/2009 (equivalent to European Union Decision 2002/657/EC). Fish samples were extracted with ethyl acetate and hexane followed by C18 solid phase clean-up and chromatographic separation on a reversed-phase C18 LC column with acetonitrile:water as mobile phase. The method results were also was compared with those obtained using liquid chromatography-tandem quadrupole mass spectrometry. The method meets the Brazilian regulatory requirements with a decision limit (CCa) of 840?mu g/kg and detection capability (CC beta) of 879?mu g/kg. This method is easy to use and has been implemented into Brazil's residue control program, with liquid chromatography-tandem mass spectrometry (LC-MS/MS) confirmation of any suspect samples using the same method. (c) 2012 John Wiley & Sons, Ltd.
dc.description4
dc.description1
dc.description125
dc.description129
dc.descriptionFood and Agriculture Organization (FAO)
dc.descriptionInternational Atomic Energy Agency (IAEA) Joint Division of Nuclear Techniques in Food and Agriculture [D5-20-36]
dc.descriptionInternational Atomic Energy Agency (IAEA) Joint Division of Nuclear Techniques in Food and Agriculture [D5-20-36]
dc.languageen
dc.publisherWiley-blackwell
dc.publisherHoboken
dc.publisherEUA
dc.relationDrug Testing And Analysis
dc.relationDrug Test. Anal.
dc.rightsfechado
dc.rightshttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.sourceWeb of Science
dc.subjecthigh performance liquid chromatography
dc.subjectresidues
dc.subjectflorfenicol
dc.subjectfish
dc.subjectliquid chromatography-tandem mass spectrometry
dc.subjectLiquid-chromatography
dc.subjectGas-chromatography
dc.subjectBovine Liver
dc.subjectAmine
dc.subjectChloramphenicol
dc.subjectThiamphenicol
dc.subjectPharmacokinetics
dc.subjectSpectrometry
dc.subjectExtraction
dc.subjectAgents
dc.titleMonitoring of florfenicol residues in fish muscle by HPLC-UV with confirmation of suspect results by LC-MS/MS
dc.typeArtículos de revistas


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