Fourier transform infrared microspectroscopy as a bacterial source tracking tool to discriminate fecal E. coli strains

dc.creatorCarlos, C
dc.creatorMaretto, DA
dc.creatorPoppi, RJ
dc.creatorSato, MIZ
dc.creatorOttoboni, LMM
dc.date2011
dc.dateSEP
dc.date2014-07-30T17:36:58Z
dc.date2015-11-26T16:45:45Z
dc.date2014-07-30T17:36:58Z
dc.date2015-11-26T16:45:45Z
dc.date.accessioned2018-03-28T23:31:23Z
dc.date.available2018-03-28T23:31:23Z
dc.identifierMicrochemical Journal. Elsevier Science Bv, v. 99, n. 1, n. 15, n. 19, 2011.
dc.identifier0026-265X
dc.identifierWOS:000292431300003
dc.identifier10.1016/j.microc.2011.03.002
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/67088
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/67088
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1274310
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionThe aim of this work was to use the FT-IR microspectroscopy technique, a union of a FT-IR spectrometer with a microscope, to discriminate fecal Escherichia coli strains from cows, chickens and humans and to compare the efficiency of this method with the genomic fingerprinting method, BOX-PCR. The obtained BOX-PCR profiles were able to correctly discriminate 93.75% of the chicken strains, 80% of the cow strains and 65% of the human strains. An efficient PLS-DA model was developed, using orthogonal signal correction and the second derivate of the FT-IR spectra. This model allowed the correct discrimination, according to the animal source, of all the E. coil strains analyzed. The bands in the FT-IR spectra that were responsible for the strains discrimination were in the region between 2816 and 3026 cm(-1) wavenumber, described as fatty acids. It was demonstrated that FT-IR microspectroscopy can be a suitable tool for fecal E. coil discrimination, because it is fast, easy to carry out and presents a flexible discrimination power. (C) 2011 Elsevier B.V. All rights reserved.
dc.description99
dc.description1
dc.description15
dc.description19
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFAPESP [FAPESP 2007/55312-6, FAPESP 2006/07309-3, 07/57025-4]
dc.descriptionCNPq [CNPq 502198/2008-7]
dc.languageen
dc.publisherElsevier Science Bv
dc.publisherAmsterdam
dc.publisherHolanda
dc.relationMicrochemical Journal
dc.relationMicrochem J.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectBacterial source tracking
dc.subjectEscherichia coli
dc.subjectBOX-PCR
dc.subjectFourier transform infrared microspectroscopy
dc.subjectRepetitive Dna-sequences
dc.subjectComposite Data Sets
dc.subjectEscherichia-coli
dc.subjectMultivariate-analysis
dc.subjectWild-animals
dc.subjectSpectroscopy
dc.subjectIdentification
dc.subjectWater
dc.subjectPcr
dc.subjectPopulations
dc.titleFourier transform infrared microspectroscopy as a bacterial source tracking tool to discriminate fecal E. coli strains
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución