Maximization of beta-Galactosidase Production: A Simultaneous Investigation of Agitation and Aeration Effects

dc.creatorAlves, FG
dc.creatorMaugeri, F
dc.creatorBurkert, JFD
dc.creatorKalil, SJ
dc.date2010
dc.dateMAR
dc.date2014-11-17T18:23:08Z
dc.date2015-11-26T16:45:37Z
dc.date2014-11-17T18:23:08Z
dc.date2015-11-26T16:45:37Z
dc.date.accessioned2018-03-28T23:31:15Z
dc.date.available2018-03-28T23:31:15Z
dc.identifierApplied Biochemistry And Biotechnology. Humana Press Inc, v. 160, n. 5, n. 1528, n. 1539, 2010.
dc.identifier0273-2289
dc.identifierWOS:000274398500025
dc.identifier10.1007/s12010-009-8683-z
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/53366
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/53366
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/53366
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1274274
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionIn this work, the agitation and aeration effects in the maximization of the beta-galactosidase production from Kluyveromyces marxianus CCT 7082 were investigated simultaneously, in relation to the volumetric enzyme activity and the productivity, as well as the analysis of the lactose consumption and production of glucose, and galactose of this process. Agitation and aeration effects were studied in a 2 L batch stirred reactor. A central composite design (2(2) trials plus three central points) was carried out. Agitation speed varied from 200 to 500 rpm and aeration rate from 0.5 to 1.5 vvm. It has been shown in this study that the volumetric enzyme production was strongly influenced by mixing conditions, while aeration was shown to be less significant. Linear models for activity and productivity due to agitation and aeration were obtained. The favorable condition was 500 rpm and 1.5 vvm, which lead to the best production of 17 U mL(-1) for enzymatic activity, 1.2 U mL(-1) h(-1) for productivity in 14 h of process, a cellular concentration of 11 mg mL(-1), and a 167.2 h(-1) volumetric oxygen transfer coefficient.
dc.description160
dc.description5
dc.description1528
dc.description1539
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.languageen
dc.publisherHumana Press Inc
dc.publisherTotowa
dc.publisherEUA
dc.relationApplied Biochemistry And Biotechnology
dc.relationAppl. Biochem. Biotechnol.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectAgitation
dc.subjectAeration
dc.subjectEnzymatic activity
dc.subjectProductivity
dc.subjectStirred reactor
dc.subjectKluyveromyces-marxianus
dc.subjectCheese Whey
dc.subjectLactose Hydrolysis
dc.subjectOxygen-transfer
dc.subjectLipase Production
dc.subjectFactorial Design
dc.subjectBatch Cultures
dc.subjectKinetic-model
dc.subjectOligosaccharides
dc.subjectGrowth
dc.titleMaximization of beta-Galactosidase Production: A Simultaneous Investigation of Agitation and Aeration Effects
dc.typeArtículos de revistas


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