eae-negative attaching and effacing Escherichia coli from piglets with diarrhea

dc.creatorPenteado, AS
dc.creatorAidar, L
dc.creatorde Castro, AFP
dc.creatorYamada, A
dc.creatorAndrade, JRC
dc.creatorBlanco, J
dc.creatorBlanco, M
dc.creatorBlanco, JE
dc.date2001
dc.dateJAN-FEB
dc.date2014-12-02T16:30:48Z
dc.date2015-11-26T16:44:58Z
dc.date2014-12-02T16:30:48Z
dc.date2015-11-26T16:44:58Z
dc.date.accessioned2018-03-28T23:30:30Z
dc.date.available2018-03-28T23:30:30Z
dc.identifierResearch In Microbiology. Editions Scientifiques Medicales Elsevier, v. 152, n. 1, n. 75, n. 81, 2001.
dc.identifier0923-2508
dc.identifierWOS:000167518500009
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/79713
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/79713
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/79713
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1274088
dc.descriptionOne hundred and ninety strains of Escherichia toll that were isolated from pigs with diarrhea in the state of Sao Paulo, Brazil, and that were negative for enterotoxins and cytotoxins were investigated. Strains which adhered to HeLa cells were examined for fluorescence actin staining (FAS), the ability to induce attaching and effacing (A/E) lesions on HEp-2 cells detectable by transmission electron microscopy and the presence of eae gene sequences detected by PCR. Intimin production was detected by western blot and serogrouping was performed. Forty-seven isolates adhered to HeLa cells in several patterns, but none adhered in a localized adherence pattern. However, seven of the 47 adherent strains were positive for the FAS reaction, although the reactions were usually weak or atypical. One FAS-negative and three FAS-positive strains, which were examined for their ability to induce A/E lesions, were all positive. Subsequently, testing of these strains for the ene gene showed that they all lacked this gene. These findings, along with earlier reports of eae-negative A/E E. coli, suggest that higher quantities of E. coli in this category might be detected if more reliance were placed on phenotypic tests rather than on gene detection tests alone. (C) 2001 Editions scientifiques et medicales Elsevier SAS.
dc.description152
dc.description1
dc.description75
dc.description81
dc.languageen
dc.publisherEditions Scientifiques Medicales Elsevier
dc.publisherParis Cedex 15
dc.publisherFrança
dc.relationResearch In Microbiology
dc.relationRes. Microbiol.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectEscherichia coli, porcine
dc.subjectdiarrhea
dc.subjectphenotypic and genotypic properties
dc.subjecteae gene
dc.subjectTissue-culture Cells
dc.subjectAdherence Patterns
dc.subjectHep-2 Cells
dc.subjectDna Probe
dc.subjectLesions
dc.subjectIdentification
dc.subjectVirulence
dc.subjectAdhesion
dc.subjectProteins
dc.subjectRabbits
dc.titleeae-negative attaching and effacing Escherichia coli from piglets with diarrhea
dc.typeArtículos de revistas


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