Artículos de revistas
Differentially Delayed Root Proteome Responses to Salt Stress in Sugar Cane Varieties
Registro en:
Journal Of Proteome Research. Amer Chemical Soc, v. 12, n. 12, n. 5681, n. 5695, 2013.
1535-3893
1535-3907
WOS:000328231300029
10.1021/pr400654a
Autor
Pacheco, CM
Pestana-Calsa, MC
Gozzo, FC
Nogueira, RJMC
Menossi, M
Calsa, T
Institución
Resumen
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Soil salinity is a limiting factor to sugar cane crop development, although in general plants present variable mechanisms of tolerance to salinity stress. The molecular basis underlying these mechanisms can be inferred by using proteomic analysis. Thus, the objective of this work was to identify differentially expressed proteins in sugar cane plants submitted to salinity stress. For that, a greenhouse experiment was established with four sugar cane varieties and two salt conditions, 0 mM (control) and 200 mM NaCl. Physiological and proteomics analyses were performed after 2 and 72 h of stress induction by salt. Distinct physiological responses to salinity stress were observed in the varieties and linked to tolerance mechanisms. In proteomic analysis, the roots soluble protein fraction was extracted, quantified, and analyzed through bidimensional electrophoresis. Gel images analyses were done computationally, where in each contrast only one variable was considered (salinity condition or variety). Differential spots were excised, digested by trypsin, and identified via mass spectrometry. The tolerant variety RB867515 showed the highest accumulation of proteins involved in growth, development, carbohydrate and energy metabolism, reactive oxygen species metabolization, protein protection, and membrane stabilization after 2 h of stress. On the other hand, the presence of these proteins in the sensitive variety was verified only in stress treatment after 72 h. These data indicate that these stress responses pathways play a role in the tolerance to salinity in sugar cane, and their effectiveness for phenotypical tolerance depends on early stress detection and activation of the coding genes expression. 12 12 5681 5695 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Financiadora de Estudos e Projetos (FINEP) National Institute of Science and Technology of Bioethanol (INCT Bioetanol) Fundacao de Amparo a Ciencia e Tecnologia do Estado de Pernambuco (FACEPE) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)