Effect of vitamin E supplementation on antibody levels against malondialdehyde modified LDL in hyperlipidemic hamsters

dc.creatorde Oliveira, FG
dc.creatorRossi, CL
dc.creatorde Oliveira, MG
dc.creatorSaad, MJA
dc.creatorVelloso, LA
dc.date2000
dc.dateAUG
dc.date2014-12-02T16:30:12Z
dc.date2015-11-26T16:43:56Z
dc.date2014-12-02T16:30:12Z
dc.date2015-11-26T16:43:56Z
dc.date.accessioned2018-03-28T23:29:07Z
dc.date.available2018-03-28T23:29:07Z
dc.identifierCardiovascular Research. Elsevier Science Bv, v. 47, n. 3, n. 567, n. 573, 2000.
dc.identifier0008-6363
dc.identifierWOS:000089056500017
dc.identifier10.1016/S0008-6363(00)00121-8
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/64004
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/64004
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/64004
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1273755
dc.descriptionObjective: The aim of this work was to investigate the effect of vitamin E (VE) supplementation on the formation of autoantibodies against oxidized low density lipoproteins (LDL) in a hyperlipidemic animal model. Methods: Thirty-four male hamsters (Mesocricetus auratus), (4 weeks old) were divided into three groups: Group A (n=9) was fed with standard rodent chow; group B (n=13) was fed with a standard rodent chow plus 2% cholesterol and 10% butter and group C (n=12) was fed with the same diet plus 0.2% (w/w) VE. Blood samples were collected by intracardiac puncture and antibody levels were determined in each animal at 4 weeks of age and after 20 weeks of experimental diet. A modified ELISA technique was used to analyze the modulation of autoantibody titers against an epitope of oxidized LDL in serum samples. Antigens prepared for the ELISA tests were characterized using spectrofluorimetry. Serum VE levels were determined in the Lipidic fractions by HPLC. Results: The groups fed with cholesterol-fat enriched diet presented a three-fold increase in total serum cholesterol and mio-fold increase in serum triglycerides compared to the control group. VE supplementation played no role in serum cholesterol and serum triglyceride concentrations but led to a decreased autoantibody (anti-LDL-malondialdehyde) formation (P<0.05). Conclusions: Our results show that VE supplementation leads to a lower production of autoantibodies against oxidized LDL, suggesting a protective effect of VE against in vivo oxidation of LDL particles, in a dose-dependent manner. (C) 2000 Published by Elsevier Science B.V.
dc.description47
dc.description3
dc.description567
dc.description573
dc.languageen
dc.publisherElsevier Science Bv
dc.publisherAmsterdam
dc.publisherHolanda
dc.relationCardiovascular Research
dc.relationCardiovasc. Res.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectatherosclerosis
dc.subjectlipoproteins
dc.subjectcholesterol
dc.subjectfree radicals
dc.subjectLow-density-lipoprotein
dc.subjectRandomized Controlled Trial
dc.subjectOxidized Ldl
dc.subjectAtherosclerotic Lesions
dc.subjectAlpha-tocopherol
dc.subjectMyocardial-infarction
dc.subjectDeficient Mice
dc.subjectAutoantibodies
dc.subjectOxidation
dc.subjectRabbits
dc.titleEffect of vitamin E supplementation on antibody levels against malondialdehyde modified LDL in hyperlipidemic hamsters
dc.typeArtículos de revistas


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