Evaluation of the inhibitory effect of amphotericin B on the apical growth of F-solani using the BioCell-Tracer((R)) system

dc.creatorTeixeira, ABA
dc.creatorMoretti, ML
dc.creatorMachado, HC
dc.creatorNishimura, K
dc.creatorTaguchi, H
dc.creatorSchreiber, AZ
dc.date2007
dc.dateMAY
dc.date2014-11-17T14:07:29Z
dc.date2015-11-26T16:43:49Z
dc.date2014-11-17T14:07:29Z
dc.date2015-11-26T16:43:49Z
dc.date.accessioned2018-03-28T23:28:56Z
dc.date.available2018-03-28T23:28:56Z
dc.identifierMycoses. Blackwell Publishing, v. 50, n. 3, n. 183, n. 188, 2007.
dc.identifier0933-7407
dc.identifierWOS:000246151400004
dc.identifier10.1111/j.0933-7407.2007.01367.x
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/65986
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/65986
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/65986
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1273708
dc.descriptionThe BioCell-Tracer (R) (BCT) system is an automatic microscopic method used for measuring the growth rate of a single fungal hyphae, which has not yet been applied to study Fusarium spp. Considering the large resistance of Fusarium species to the available chemotherapy and that hyphae is the morphological fungal form most often seen in vivo, in this work, Amphotericin B MIC and MFC values for a Fusarium solani strain were obtained by the conventional assay method testing conidia and also by the BCT (R) monitoring system. Both MIC and MFC values of AMB against F. solani determined by broth dilution method resulted in 4.0 mu g ml(-1). By the BCT (R) system, their values were 1.0 mu g ml(-1), with an inhibition rate of 99.5% (Exp-GR) and 100.0% (Post-GR), showing that when testing hyphae directly, MIC and MFC were determined at two lower dilutions than the MIC and MFC values obtained with conidia. Using the BCT (R) system, 4.0, 2.0 and 1.0 mu g ml(-1) of AMB concentrations inhibited hyphae growth in 50 min whereas 0.5 mu g ml(-1) of AMB needed 100 min to start hyphae growth inhibition. These findings lead us to conclude that antifungal susceptibility varies between conidia and hyphae. For this strain of Fusarium solani, hyphae were more susceptible to AMB than conidia.
dc.description50
dc.description3
dc.description183
dc.description188
dc.languageen
dc.publisherBlackwell Publishing
dc.publisherOxford
dc.publisherInglaterra
dc.relationMycoses
dc.relationMycoses
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectAmphotericin B
dc.subjectBioCell-tracer
dc.subjectFusarium sp
dc.subjectsusceptibility test
dc.subjectSingle Hypha
dc.subjectAntifungal Activity
dc.subjectFilamentous Fungi
dc.subjectAgents
dc.titleEvaluation of the inhibitory effect of amphotericin B on the apical growth of F-solani using the BioCell-Tracer((R)) system
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución