Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)The purpose of this work was to evaluate the incorporation of conjugated linoleic and -linolenic fatty acids into muscle tissue of genetically modified tilapia through treatment with CLA and tung oil-enriched diet as a source of these acids by gas chromatograph equipped with a FID (GC-FID). The results showed that conjugated fatty acids were incorporated into tilapia muscle tissue and the accumulation had higher values in fourth period, except the -eleostearic acid. Furthermore, other fatty acids of important nutritional value such as EPA, DHA, and AA acids were also identified in this study. Tilapia treated with diets enriched with CLA and tung oil can be included in human diet as a source of conjugated fatty acids such as conjugated linoleic and -linolenic acids. Practical applications: Adding CLA and tung oil, a source of conjugated -linolenic acid, to tilapia feed, can significantly increase the concentration of conjugated fatty acids in tilapia muscle tissue. Tilapia fed with CLA and CLnA can be included in human diet as a source of conjugated fatty acids.1151011391145Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)