Determination of oxytetracycline in tomatoes by HPLC using fluorescence detection

dc.creatorMaia, PP
dc.creatorRath, S
dc.creatorReyes, FG
dc.date2008
dc.date37073
dc.date2014-11-17T12:39:42Z
dc.date2015-11-26T16:43:03Z
dc.date2014-11-17T12:39:42Z
dc.date2015-11-26T16:43:03Z
dc.date.accessioned2018-03-28T23:27:55Z
dc.date.available2018-03-28T23:27:55Z
dc.identifierFood Chemistry. Elsevier Sci Ltd, v. 109, n. 1, n. 212, n. 218, 2008.
dc.identifier0308-8146
dc.identifierWOS:000254597100030
dc.identifier10.1016/j.foodchem.2007.12.019
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/79563
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/79563
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/79563
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1273469
dc.descriptionAn analytical method for the determination of oxytetracycline (OTC) in tomatoes was developed and validated. Liquid-liquid extraction (LLE) and a solid-phase extraction (SPE) were used for sample preparation. Reversed-phase high performance liquid chromatography (FP-HPLC) using a C-18 column and a mobile phase containing MeOH: calcium chloride, disodium ethylenediaminetetraacetate (EDTA) and sodium acetate, pH 7.3 (30:70, v/v), with fluorescence detection at 390 nm excitation and 512 nm emission, was used for separation and quantitation of OTC. The method was validated through the following performance criteria: linearity and linear range, sensitivity, selectivity, intra-day and inter-day precision, detection and quantitation limits and accuracy. Limit of quantitation show that the method developed is suitable for the determination of OTC at a level below the maximum residue limits established by the Brazilian legislations (250 mu g kg(-1)). Of 40 samples analyzed, none contained OTC above the limit of quantitation. (C) 2007 Elsevier Ltd. All rights reserved.
dc.description109
dc.description1
dc.description212
dc.description218
dc.languageen
dc.publisherElsevier Sci Ltd
dc.publisherOxford
dc.publisherInglaterra
dc.relationFood Chemistry
dc.relationFood Chem.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjecttomatoes
dc.subjectantibiotics
dc.subjectoxytetracycline
dc.subjectmethod validation
dc.subjecthigh performance liquid chromatography
dc.subjectPerformance Liquid-chromatography
dc.subjectTetracycline Antibiotics
dc.subjectChlortetracycline
dc.titleDetermination of oxytetracycline in tomatoes by HPLC using fluorescence detection
dc.typeArtículos de revistas


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