Determination of salicylate in blood serum by flow injection with immobilized salicylate hydroxylase

dc.creatorVila, MMDC
dc.creatorTubino, M
dc.creatorNeto, GD
dc.date2001
dc.dateSEP-OCT
dc.date2014-07-30T14:39:35Z
dc.date2015-11-26T16:42:55Z
dc.date2014-07-30T14:39:35Z
dc.date2015-11-26T16:42:55Z
dc.date.accessioned2018-03-28T23:27:41Z
dc.date.available2018-03-28T23:27:41Z
dc.identifierJournal Of Aoac International. Aoac International, v. 84, n. 5, n. 1363, n. 1369, 2001.
dc.identifier1060-3271
dc.identifierWOS:000171421000010
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/61419
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/61419
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1273430
dc.descriptionA flow injection (FI) enzymatic system, based on the use of immobilized salicylate hydroxylase in glass beads, was developed for the determination of salicylate. Salicylate hydroxylase and nicotinamide adenine dinucleotide (NADH) are used to convert salicylate to catechol. The reaction of catechol with 4-aminophenol at high pH yields a colored product which is detected spectrophotometrically at 565 nm. Ten samples of human serum containing from 5.0 x 10(-4) to 5.0 x 10(-3) mol/L added salicylate were analyzed and the recovery was determined. Eight additional serum samples containing salicylate were analyzed by the Trinder test and the proposed method. The results obtained with the 2 methods showed good agreement by the statistical Student's t-test. The relative precision of the method is about 3.4% (RSD of the mean recovery). Considering the lowest concentration analyzed, the quantitative limit of detection is about 0.2 x 10(-5) mol/L (3 x SD). The volume of the sample used was 150 muL. The proposed method was also used to analyze medicines containing acetylsalicylic acid. The results were statistically compared with those obtained through the U.S. Pharmacopoeia procedure and showed excellent agreement.
dc.description84
dc.description5
dc.description1363
dc.description1369
dc.languageen
dc.publisherAoac International
dc.publisherGaithersburg
dc.publisherEUA
dc.relationJournal Of Aoac International
dc.relationJ. AOAC Int.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectSynchronous Fluorescence Spectrometry
dc.subjectPerformance Liquid-chromatography
dc.subjectAcetylsalicylic-acid
dc.subjectAspirin
dc.subjectPlasma
dc.subjectElectrode
dc.subjectConstruction
dc.subjectMetabolites
dc.subjectBiosensor
dc.subjectSensor
dc.titleDetermination of salicylate in blood serum by flow injection with immobilized salicylate hydroxylase
dc.typeArtículos de revistas


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