Blood group genotyping facilitates transfusion of beta-thalassemia patients

dc.creatorCastilho, L
dc.creatorRios, M
dc.creatorPellegrino, J
dc.creatorSaad, STO
dc.creatorCosta, FF
dc.date2002
dc.date2014-07-30T13:52:29Z
dc.date2015-11-26T16:42:35Z
dc.date2014-07-30T13:52:29Z
dc.date2015-11-26T16:42:35Z
dc.date.accessioned2018-03-28T23:27:11Z
dc.date.available2018-03-28T23:27:11Z
dc.identifierJournal Of Clinical Laboratory Analysis. Wiley-liss, v. 16, n. 5, n. 216, n. 220, 2002.
dc.identifier0887-8013
dc.identifierWOS:000178402900002
dc.identifier10.1002/jcla.10044
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/55784
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/55784
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1273303
dc.descriptionWe evaluated the usefulness of blood group genotyping as a supplement to hemagglutination to determine the red blood cell (RBC) antigen profile of polytransfused patients with beta-thalassemia. We selected 10 alloimmunized patients who were receiving antigen-matched RBCs based on phenotype, and had clinical evidence of delayed hemolytic transfusion reaction. DNA was prepared from blood samples and RH E/e, K1/K2, FY A/FY B, and X A/JK B alleles were determined by PCR-RFLP. RH D/non-D was determined according to the PCR product size associated with the RHD gene sequence in intron 4 and exon 10/3'UTR. RH C/c was tested by multiplex PCR. The phenotypes and genotypes of nine of the 10 samples were discrepant. Five of the discrepancies occurred in the Rh system. One sample was phenotyped as Rhcc and genotyped as RH C/C, and two samples were phenotyped as RhCc and genotyped as RH C/C. Two other samples were phenotyped as RhEe and genotyped as RH e/e. Three samples had discrepancies in the Kidd system with phenotype Jk(a+b+) and were genotyped as homozygous for JK B. One sample had a discrepancy in the Duffy system: it was phenotyped as Fy(a+b-) and homozygous for FY B. Genotyping was very important in determining the true blood groups of many polytransfused patients with beta-thalassemia, and it assisted in the identification of suspected alloantibodies and the selection of antigen-negative RBCs for transfusion. (C) 2002 Wiley-Liss, Inc.
dc.description16
dc.description5
dc.description216
dc.description220
dc.languageen
dc.publisherWiley-liss
dc.publisherNew York
dc.publisherEUA
dc.relationJournal Of Clinical Laboratory Analysis
dc.relationJ. Clin. Lab. Anal.
dc.rightsfechado
dc.rightshttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.sourceWeb of Science
dc.subjectgenotyping assays
dc.subjectblood group antigens
dc.subjecthemagglutination
dc.subjecttransfusion
dc.subjectmicrochimerism
dc.subjectRed-cell Alloantibodies
dc.subjectMultiple Transfusions
dc.subjectGroup Antigens
dc.subjectAlloimmunization
dc.subjectRhd
dc.subjectTherapy
dc.subjectSamples
dc.subjectRhce
dc.titleBlood group genotyping facilitates transfusion of beta-thalassemia patients
dc.typeArtículos de revistas


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