Detection and possible prognostic relevance of p53 gene mutations in diffuse large B-cell lymphoma. An analysis of 51 cases and review of the literature

dc.creatorKerbauy, FR
dc.creatorColleoni, GWB
dc.creatorSaad, STO
dc.creatorSilva, MRR
dc.creatorAlves, AC
dc.creatorAguiar, KCC
dc.creatorAlbuquerque, DM
dc.creatorKobarg, J
dc.creatorSeixas, MT
dc.creatorKerbauy, J
dc.date2004
dc.dateOCT
dc.date2014-07-30T14:38:54Z
dc.date2015-11-26T16:42:22Z
dc.date2014-07-30T14:38:54Z
dc.date2015-11-26T16:42:22Z
dc.date.accessioned2018-03-28T23:26:53Z
dc.date.available2018-03-28T23:26:53Z
dc.identifierLeukemia & Lymphoma. Taylor & Francis Ltd, v. 45, n. 10, n. 2071, n. 2078, 2004.
dc.identifier1042-8194
dc.identifierWOS:000223523600014
dc.identifier10.1080/10428190410001713170
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/61285
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/61285
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1273246
dc.descriptionDiffuse large B-cell lymphoma (DLBCL) is the most common type of non-Hodgkin's lymphoma. Although the presence of p53 gene mutations has been considered as a bad prognostic feature in DLBCL, its clinical significance is still controversial. The aims of this study were: detect the presence of mutations in exons 5 to 9 of the p53 gene and correlate it to prognosis in DLBCL. Fifty-one DLBCL patients were enrolled in this study. Expression of p53 was evaluated by immunohistochemistry. The screening of p53 mutations was performed using PCR-SSCP methods. Cases showing a mobility shift on SSCP electrophoresis were analyzed by automatic sequencing. We could identify 8 missense mutations in 6 of 48 cases (12.5%). In addition, we found a known polymorphism at codon 213 and 2 instances of silent mutations. Of all mutations/polymorphisms found, 7 (64%) were localized in codons previously described as p53 hot spots in NHL cases. Of the remaining alterations (4 or 36%), 2 mutations were localized in codons previously described as hot spots for p53 in other tumors and 2 (codon 142 of the exon 5 and codon 195 of the exon 6), in codons not described as hot spots for p53 up to now. The presence of missense mutations in exons 5 to 9 of p53 gene had adverse impact on overall survival (P=0.020). Cox's Regression Model identified that high-risk International Prognostic Index (IPI) and p53 gene mutations have independent negative impact on OS. Therefore, the association of IPI with cellular factors, such as p53 mutation, can be very helpful in deciding when we should indicate more aggressive therapies in patients with DLBCL, to somehow increase the chance of cure in these patients.
dc.description45
dc.description10
dc.description2071
dc.description2078
dc.languageen
dc.publisherTaylor & Francis Ltd
dc.publisherAbingdon
dc.publisherInglaterra
dc.relationLeukemia & Lymphoma
dc.relationLeuk. Lymphoma
dc.rightsfechado
dc.rightshttp://journalauthors.tandf.co.uk/permissions/reusingOwnWork.asp
dc.sourceWeb of Science
dc.subjectnon-Hodgkin's lymphomas
dc.subjectP53 protein
dc.subjectp53 gene mutation
dc.subjectprognosis
dc.subjectNon-hodgkins-lymphomas
dc.subjectCrystal-structure
dc.subjectBcl-2 Expression
dc.subjectHuman Cancer
dc.subjectSurvival
dc.subjectChemotherapy
dc.subjectGrade
dc.subjectMdm2
dc.subjectP21
dc.titleDetection and possible prognostic relevance of p53 gene mutations in diffuse large B-cell lymphoma. An analysis of 51 cases and review of the literature
dc.typeArtículos de revistas


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