Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Brazil nut oil is one of the important Amazonian natural products in the global market. Despite its health benefits and applications in food and cosmetic industries, authentication and quality control of the oil are far from satisfactory. Several sample's of Amazonian Brazil nut oil (authentic oils of different geographic origins,, commercial Oil's; and oils adulterated with soybean oil) were evaluated by easy. ambient sonic spray ionization mass spectrometry (EASI-MS). The samples were characterized on the basis of triacylglycerol profiles, and their major ions. were subjected to chemometric treatment (principal component analysis). The ambient mass spectrometry analysis and the set of major bans allowed the characterization of authentic oils, commercial oils, and adulterated oils (5% soybean oil) in a few minutes and without sample preparation. Therefore, it is shown that quality control of Amazonian vegetable oils can be readily accomplished by EASI-MS.60451126311267Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Brazilian Science Foundation FAPEAMBrazilian Science Foundation INCT/CEABFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)