Isolation and characterization of alachlor-degrading actinomycetes from soil

dc.creatorSette, LD
dc.creatorde Oliveira, VM
dc.creatorManfio, GP
dc.date2005
dc.dateFEB
dc.date2014-11-17T10:49:13Z
dc.date2015-11-26T16:42:19Z
dc.date2014-11-17T10:49:13Z
dc.date2015-11-26T16:42:19Z
dc.date.accessioned2018-03-28T23:26:48Z
dc.date.available2018-03-28T23:26:48Z
dc.identifierAntonie Van Leeuwenhoek International Journal Of General And Molecular Microbiology. Springer, v. 87, n. 2, n. 81, n. 89, 2005.
dc.identifier0003-6072
dc.identifierWOS:000228392000001
dc.identifier10.1007/s10482-004-1129-2
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/60803
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/60803
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/60803
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1273223
dc.descriptionAlachlor (2-cloro-N-(methoxymethyl)-N-(2,6-diethylphenyl)-acetamide) is an extremely toxic and highly mobile herbicide that is widely used for pre-emergence control of grasses and weeds in many commercial crops in Brazil. In order to select soil actinomycetes able to degrade this herbicide, fifty-three actinomycete strains were isolated from soil treated with alachlor using selective conditions and subjected to in vitro degradation assays. Sixteen isolates were shown to be tolerant to high concentrations of the herbicide (up to 720 mg L-1), and six of these were able to grow and degrade >= 50% alachlor (72 mg L-1) in mineral salts medium. Morphological and phylogenetic analysis enabled the assignment of the alachlor-degrading strains to the genus Streptomyces. Strain LS151 was related to the type strains of Streptomyces capoamus/Streptomyces galbus, whereas strains LS143 and LS153 were related to Streptomyces bikiniensis. The remaining strains, LS166, LS177 and LS182, were similar in morphological features and recovered in a single cluster based on 16S rDNA sequence analysis, but shown to be distinct on the, basis of genomic fingerprint data (rep-PCR). Though a definitive taxonomic assignment of alachlor-degrading strains was not possible, these data indicate that ability to degrade this pesticide was detected in different Streptomyces taxa.
dc.description87
dc.description2
dc.description81
dc.description89
dc.languageen
dc.publisherSpringer
dc.publisherDordrecht
dc.publisherHolanda
dc.relationAntonie Van Leeuwenhoek International Journal Of General And Molecular Microbiology
dc.relationAntonie Van Leeuwenhoek
dc.rightsfechado
dc.rightshttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.sourceWeb of Science
dc.subject16S rDNA
dc.subjectalachlor
dc.subjectbiodegradation
dc.subjectchloroacetanilide herbicide
dc.subjectsoil streptomycetes
dc.subjectDna
dc.subjectBiodegradation
dc.subjectMetolachlor
dc.subjectStrategies
dc.subjectHerbicides
dc.subjectSequences
dc.subjectGenomes
dc.titleIsolation and characterization of alachlor-degrading actinomycetes from soil
dc.typeArtículos de revistas


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