Biostimulatory effects of low-level laser therapy on epithelial cells and gingival fibroblasts treated with zoledronic acid

dc.creatorBasso, FG
dc.creatorPansani, TN
dc.creatorTurrioni, APS
dc.creatorKurachi, C
dc.creatorBagnato, VS
dc.creatorHebling, J
dc.creatorCosta, CAD
dc.date2013
dc.dateMAY
dc.date2014-07-30T13:52:20Z
dc.date2015-11-26T16:41:51Z
dc.date2014-07-30T13:52:20Z
dc.date2015-11-26T16:41:51Z
dc.date.accessioned2018-03-28T23:26:11Z
dc.date.available2018-03-28T23:26:11Z
dc.identifierLaser Physics. Iop Publishing Ltd, v. 23, n. 5, 2013.
dc.identifier1054-660X
dc.identifierWOS:000318007600025
dc.identifier10.1088/1054-660X/23/5/055601
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/55699
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/55699
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1273065
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionLow-level laser therapy (LLLT) has been considered as an adjuvant treatment for bisphosphonate-related osteonecrosis, presenting positive clinical outcomes. However, there are no data regarding the effect of LLLT on oral tissue cells exposed to bisphosphonates. This study aimed to evaluate the effects of LLLT on epithelial cells and gingival fibroblasts exposed to a nitrogen-containing bisphosphonate-zoledronic acid (ZA). Cells were seeded in wells of 24-well plates, incubated for 48 h and then exposed to ZA at 5 mu M for an additional 48 h. LLLT was performed with a diode laser prototype-LaserTABLE (InGaAsP-780 nm +/- 3 nm, 25 mW), at selected energy doses of 0.5, 1.5, 3, 5, and 7 J cm(-2) in three irradiation sessions, every 24 h. Cell metabolism, total protein production, gene expression of vascular endothelial growth factor (VEGF) and collagen type I (Col-I), and cell morphology were evaluated 24 h after the last irradiation. Data were statistically analyzed by Kruskal-Wallis and Mann-Whitney tests at 5% significance. Selected LLLT parameters increased the functions of epithelial cells and gingival fibroblasts treated with ZA. Gene expression of VEGF and Col-I was also increased. Specific parameters of LLLT biostimulated fibroblasts and epithelial cells treated with ZA. Analysis of these in vitro data may explain the positive in vivo effects of LLLT applied to osteonecrosis lesions.
dc.description23
dc.description5
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFAPESP [2009/54722-1, BP.DR 2009/52326-1]
dc.descriptionCNPq [301029/2010-1]
dc.languageen
dc.publisherIop Publishing Ltd
dc.publisherBristol
dc.publisherInglaterra
dc.relationLaser Physics
dc.relationLaser Phys.
dc.rightsaberto
dc.rightshttp://iopscience.iop.org/page/copyright
dc.sourceWeb of Science
dc.subjectBisphosphonate-associated Osteonecrosis
dc.subjectOdontoblast-like Cells
dc.subjectMultiple-myeloma
dc.subjectEndothelial-cells
dc.subjectSurgical Laser
dc.subjectRisk-factors
dc.subjectJaw
dc.subjectIrradiation
dc.subjectWavelengths
dc.subjectManagement
dc.titleBiostimulatory effects of low-level laser therapy on epithelial cells and gingival fibroblasts treated with zoledronic acid
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución