Bioluminescent yeast estrogen assay (BLYES) as a sensitive tool to monitor surface and drinking water for estrogenicity

dc.creatorBergamasco, AMD
dc.creatorEldridge, M
dc.creatorSanseverino, J
dc.creatorSodre, FF
dc.creatorMontagner, CC
dc.creatorPescara, IC
dc.creatorJardim, WF
dc.creatorUmbuzeiro, GD
dc.date2011
dc.dateNOV
dc.date2014-07-30T13:52:11Z
dc.date2015-11-26T16:41:22Z
dc.date2014-07-30T13:52:11Z
dc.date2015-11-26T16:41:22Z
dc.date.accessioned2018-03-28T23:25:34Z
dc.date.available2018-03-28T23:25:34Z
dc.identifierJournal Of Environmental Monitoring. Royal Soc Chemistry, v. 13, n. 11, n. 3288, n. 3293, 2011.
dc.identifier1464-0325
dc.identifier1464-0333
dc.identifierWOS:000297527800036
dc.identifier10.1039/c1em10464k
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/55617
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/55617
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1272913
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptiondEstrogenic Endocrine Disrupting Chemicals (EDCs) are a concern due to their ubiquity and recognized adverse effects to humans and wildlife. Methods to assess exposure to and associated risks of their presence in aquatic environment are still under development. The aim of this work is to assess estrogenicity of raw and treated waters with different degrees of pollution. Chemical analyses of selected EDCs were performed by liquid chromatography-tandem mass spectrometry, and estrogenic activity was evaluated using in vitro bioluminescent yeast estrogen assay (BLYES). Most raw water samples (18/20) presented at least one EDC and 16 rendered positive in BLYES. When EDCs were detected, the bioassay usually provided a positive response, except when only bisphenol A was detected at low concentrations. The highest values of estrogenic activity were detected in the most polluted sites. The maximum estrogenic activity observed was 8.7 ng equiv. of E2 L-1. We compared potencies observed in the bioassay to the relative potency of target compounds and their concentrations failed to fully explain the biological response. This indicates that bioassay is more sensitive than the chemical approach either detecting estrogenic target compounds at lower concentrations, other non-target compounds or even synergistic effects, which should be considered on further investigations. We have not detected either estrogenic activity or estrogenic compounds in drinking water. BLYES showed good sensitivity with a detection limit of 0.1 ng equiv. E2 L-1 and it seems to be a suitable tool for water monitoring.
dc.descriptiono TEXTO COMPLETO DESTE ARTIGO, ESTARÁ DISPONÍVEL À PARTIR DE AGOSTO DE 2015.
dc.description13
dc.description11
dc.description3288
dc.description3293
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFAPESP [2007/58449-2]
dc.languageen
dc.publisherRoyal Soc Chemistry
dc.publisherCambridge
dc.publisherInglaterra
dc.relationJournal Of Environmental Monitoring
dc.relationJ. Environ. Monit.
dc.rightsembargo
dc.sourceWeb of Science
dc.subjectTreatment-plant Effluents
dc.subjectIn-vitro Assessment
dc.subjectParis Area France
dc.subjectWaste-water
dc.subjectEndocrine Disruptors
dc.subjectEmerging Contaminants
dc.subjectVivo
dc.subjectChemicals
dc.subjectBioassays
dc.subjectSewage
dc.titleBioluminescent yeast estrogen assay (BLYES) as a sensitive tool to monitor surface and drinking water for estrogenicity
dc.typeArtículos de revistas


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