Behaviour of fibroblastic cells cultured in collagen 1 using the sandwich technique

dc.creatorHaas, VR
dc.creatorSantos, AR
dc.creatorWada, MLF
dc.date2001
dc.date2014-07-30T13:51:47Z
dc.date2015-11-26T16:41:07Z
dc.date2014-07-30T13:51:47Z
dc.date2015-11-26T16:41:07Z
dc.date.accessioned2018-03-28T23:25:14Z
dc.date.available2018-03-28T23:25:14Z
dc.identifierCytobios. Faculty Press, v. 106, n. 255, n. 267, 2001.
dc.identifier0011-4529
dc.identifierWOS:000170828500011
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/55390
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/55390
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1272825
dc.descriptionVero cells, a fibroblastic lineage derived from fibroblastic kidney cells of the African green monkey, were cultivated by means of the sandwich technique, involving glass coverslip/collagen and collagen/collagen with varied foetal calf serum concentrations in the culture medium. The cells, cultured on coverslips, then received a type I collagen gel layer on top, and migrated from the coverslip to the collagen layer. When the cells were cultivated on collagen followed by a covering of type I collagen gel, the cells migrated into both collagen layers. Cellular morphology was similar, independent of the type of sandwich and serum concentration used. Cells in contact with collagen either migrated into the layer or formed a basal lamina separating them from the collagen matrix. The formation of a basal lamina with laminin and collagen IV deposition was most noticeable when 20% foetal calf serum was used in the culture medium. Cellular infiltration into the collagen gel was most evident with the use of 10% foetal calf serum. The gel contraction was similar for the two serum concentrations employed.
dc.description106
dc.description2
dc.description255
dc.description267
dc.languageen
dc.publisherFaculty Press
dc.publisherCambridge
dc.publisherInglaterra
dc.relationCytobios
dc.relationCytobios
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectcell culture
dc.subjectextracellular matrix
dc.subjectsandwich conformation
dc.subjectcell differentiation
dc.subjectVero cells
dc.subjectLong-term Culture
dc.subjectExtracellular-matrix
dc.subjectHepatocyte Function
dc.subjectIn-vitro
dc.subject3-dimensional Matrix
dc.subjectEndothelial-cells
dc.subjectSkin Fibroblasts
dc.subjectGel Contraction
dc.subjectRat Hepatocytes
dc.subjectGrowth-factor
dc.titleBehaviour of fibroblastic cells cultured in collagen 1 using the sandwich technique
dc.typeArtículos de revistas


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