Effects of veratrine and veratridine on oxygen consumption and electrical membrane potential of isolated rat skeletal muscle and liver mitochondria

dc.creatorFreitas, EMS
dc.creatorFagian, MM
dc.creatorHofling, MAD
dc.date2006
dc.date37043
dc.date2014-11-17T05:40:22Z
dc.date2015-11-26T16:39:25Z
dc.date2014-11-17T05:40:22Z
dc.date2015-11-26T16:39:25Z
dc.date.accessioned2018-03-28T23:23:02Z
dc.date.available2018-03-28T23:23:02Z
dc.identifierToxicon. Pergamon-elsevier Science Ltd, v. 47, n. 7, n. 780, n. 787, 2006.
dc.identifier0041-0101
dc.identifierWOS:000238466300009
dc.identifier10.1016/j.toxicon.2006.02.009
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/79803
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/79803
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/79803
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1272469
dc.descriptionWe have previously shown that veratrine, a mixture of alkaloids known as Veratrum alkaloids, produces skeletal muscle toxicity, and there is evidence that veratrine interferes with the energetics of various systems, including cardiomyocytes and synaptosomes. In this work, we explored the effects of veratrine and veratridine, a component of this mixture, in rat skeletal muscle mitochondria and compared the results with those seen in liver mitochondria. Veratrine and veratridine alkaloids caused a significant concentration-dependent decrease in the rate of state 3 respiration, respiratory control (RCR) and ADP/O ratios in isolated rat skeletal muscle mitochondria (RMM), but not in rat liver mitochondria (RLM) supported by either NADH-linked substrates or succinate. The oxygen consumption experiments showed that RMM were more susceptible to the toxic action of Veratrum alkaloids than RLM. The addition of veratrine (250 mu g/ml) to RMM caused dissipation of the mitochondrial electrical membrane potential during succinate oxidation, but this effect was totally reversed by adding ATP. These results indicate that there are chemical- and tissue-specific toxic effects of veratrine and veratridine on mitochondrial respiratory chain complexes. Identification of the specific respiratory chain targets involved should provide a better understanding of the molecular mechanisms of the toxicity of these agents. (c) 2006 Elsevier Ltd. All rights reserved.
dc.description47
dc.description7
dc.description780
dc.description787
dc.languageen
dc.publisherPergamon-elsevier Science Ltd
dc.publisherOxford
dc.publisherInglaterra
dc.relationToxicon
dc.relationToxicon
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectveratrine
dc.subjectveratridine
dc.subjectisolated mitochondria
dc.subjectoxygen consumption
dc.subjectmitochondrial electrical membrane potential
dc.subjectrespiratory chain complex
dc.subjectPermeability Transition
dc.subjectOxidative-phosphorylation
dc.subjectHeart-mitochondria
dc.subjectCreatine-kinase
dc.subjectSodium-channel
dc.subjectInhibition
dc.subjectFibers
dc.subjectDrug
dc.titleEffects of veratrine and veratridine on oxygen consumption and electrical membrane potential of isolated rat skeletal muscle and liver mitochondria
dc.typeArtículos de revistas


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