dc.creatorCatisti, R
dc.creatorVercesi, AE
dc.date1999
dc.dateDEC 24
dc.date2014-12-02T16:29:23Z
dc.date2015-11-26T16:39:21Z
dc.date2014-12-02T16:29:23Z
dc.date2015-11-26T16:39:21Z
dc.date.accessioned2018-03-28T23:22:56Z
dc.date.available2018-03-28T23:22:56Z
dc.identifierFebs Letters. Elsevier Science Bv, v. 464, n. 41671, n. 97, n. 101, 1999.
dc.identifier0014-5793
dc.identifierWOS:000084654700019
dc.identifier10.1016/S0014-5793(99)01677-4
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/72207
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/72207
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/72207
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1272460
dc.descriptionThe ability of low concentrations (5-15 mu M) of long-chain fatty acids to open the permeability transition pore (PTP) in Ca2+-loaded mitochondria has been ascribed to their protonophoric effect mediated by mitochondrial anion carriers, as well as to a direct interaction with the pore assembly [M.R. Wieckowski and L. Wojtczak, FEES Lett, 423 (1998) 339-342], Here, we have compared the PTP opening ability of arachidonic acid (AA) with that of carbonyl cyanide-p-trifluoromethoxyphenylhydrazone (FCCP) at concentrations that cause similar quantitative dissipation of the membrane potential (Delta Psi) in Ca2+-loaded rat liver mitochondria respiring on succinate, The initial protonophoric effects of AA and FCCP were only slightly modified by carboxyatractyloside and were followed by PTP opening, as indicated by a second phase of Delta Psi disruption sensitive to EGTA, ADP, dithiothreitol and cyclosporin A. This second phase of Delta Psi dissipation could also be prevented by rotenone or NAD(P)H-linked substrates which decrease the pyridine nucleotide (PN) oxidation that follows the stimulation of oxygen consumption induced by AA or FCCP, These results suggest that, under the experimental conditions used here, the PTP opening induced by AA or FCCP was a consequence of PN oxidation. Exogenous catalase also inhibited both AA- and FCCP-induced PTP opening. These results indicate that a condition of oxidative stress associated with the oxidized state of PN underlies membrane protein thiol oxidation and PTP opening. (C) 1999 Federation of European Biochemical Societies.
dc.description464
dc.description41671
dc.description97
dc.description101
dc.languageen
dc.publisherElsevier Science Bv
dc.publisherAmsterdam
dc.publisherHolanda
dc.relationFebs Letters
dc.relationFEBS Lett.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectarachidonic acid
dc.subjectmitochondrion
dc.subjectmembrane permeability transition
dc.subjectNAD(P)H
dc.subjectcalcium ion
dc.subjectCa-2+ Efflux
dc.subjectOxidative-phosphorylation
dc.subjectMembrane
dc.subjectMechanism
dc.subjectTranshydrogenase
dc.subjectApoptosis
dc.subjectPore
dc.titleThe participation of pyridine nucleotides redox state and reactive oxygen in the fatty acid-induced permeability transition in rat liver mitochondria
dc.typeArtículos de revistas


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