Effects of Yersinia enterocolitica O : 3 derivatives on B lymphocyte activation in vivo

dc.creatorCrespo, ADC
dc.creatorFalcao, DP
dc.creatorde Araujo, PMF
dc.creatorde Medeiros, BMM
dc.date2002
dc.date2014-11-17T05:19:04Z
dc.date2015-11-26T16:39:03Z
dc.date2014-11-17T05:19:04Z
dc.date2015-11-26T16:39:03Z
dc.date.accessioned2018-03-28T23:22:35Z
dc.date.available2018-03-28T23:22:35Z
dc.identifierMicrobiology And Immunology. Center Academic Publ Japan, v. 46, n. 2, n. 95, n. 100, 2002.
dc.identifier0385-5600
dc.identifierWOS:000173738600006
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/79819
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/79819
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/79819
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1272380
dc.descriptionThe potential sequelae of intestinal infection with Yersinia enterocolitica include reactive arthritis, erythema nodosum, Reiter's syndrome and other autoimmune diseases. The role of the immune response in the pathogenesis of these diseases has not been fully defined, but autoimmune manifestations may be a consequence of the increase in autoantibodies as a result of polyclonal B-cell activation induced by Yersinia. We investigated the effects of Y enterocolitica 0:3 derivatives on B lymphocyte activation in vivo. Groups of five specific pathogen free (SPF) Swiss mice were inoculated with bacterial cell extract, Yersinia outermembrane proteins (Yops) or lipopolysaccharide (LPS) obtained from Y enterocolitica 0:3 and their immunoglobulin-secreting spleen cells were detected by isotype-specific protein A plaque assay. The presence of specific anti-Yersinia antibodies and autoantibodies was determined in mouse sera by ELISA. In all experiments a marked increase in the number of secretory cells of different isotypes was observed as early as the third day after inoculation. IgG and IgM anti-Yersinia antibodies were detected in the sera of all inoculated mice, and autoantibodies against myosin in the sera of those inoculated with bacterial cell extract. The sera from animals stimulated with LPS reacted with myelin, actin and laminin, while the sera from mice inoculated with Yops reacted with myelin, thyroglobulin and cardiolipin. These results suggest that SPF Swiss mice inoculated with any one of the Y enterocolitica derivatives tested exhibited polyclonal activation of B lymphocytes as a result of stimulation by various bacterial components and not only LPS stimulation.
dc.description46
dc.description2
dc.description95
dc.description100
dc.languageen
dc.publisherCenter Academic Publ Japan
dc.publisherTokyo
dc.publisherJapão
dc.relationMicrobiology And Immunology
dc.relationMicrobiol. Immunol.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectYersinia enterocolitica
dc.subjectLPS
dc.subjectyops
dc.subjectpolyclonal activation
dc.subjectTriggered Reactive Arthritis
dc.subjectThyrotropin Receptor
dc.subjectEndotoxin Protein
dc.subjectEscherichia-coli
dc.subjectOuter Membrane
dc.subjectSynovial-fluid
dc.subjectLipoprotein
dc.subjectAntibodies
dc.subjectCells
dc.subjectLipopolysaccharide
dc.titleEffects of Yersinia enterocolitica O : 3 derivatives on B lymphocyte activation in vivo
dc.typeArtículos de revistas


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