Isobaric Tagging-Based Quantification for Proteomic Analysis: A Comparative Study of Spared and Affected Muscles from mdx Mice at the Early Phase of Dystrophy

dc.creatorMatsumura, CY
dc.creatorde Oliveira, BM
dc.creatorDurbeej, M
dc.creatorMarques, MJ
dc.date2013
dc.date43252
dc.date2014-07-30T14:35:29Z
dc.date2015-11-26T16:39:00Z
dc.date2014-07-30T14:35:29Z
dc.date2015-11-26T16:39:00Z
dc.date.accessioned2018-03-28T23:22:31Z
dc.date.available2018-03-28T23:22:31Z
dc.identifierPlos One. Public Library Science, v. 8, n. 6, 2013.
dc.identifier1932-6203
dc.identifierWOS:000320576400023
dc.identifier10.1371/journal.pone.0065831
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/60784
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/60784
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1272361
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionDuchenne muscular dystrophy (DMD) is the most common childhood myopathy, characterized by muscle loss and cardiorespiratory failure. While the genetic basis of DMD is well established, secondary mechanisms associated with dystrophic pathophysiology are not fully clarified yet. In order to obtain new insights into the molecular mechanisms of muscle dystrophy during earlier stages of the disease, we performed a comparative proteomic profile of the spared extraocular muscles (EOM) vs. affected diaphragm from the mdx mice, using a label based shotgun proteomic approach. Out of the 857 identified proteins, 42 to 62 proteins had differential abundance of peptide ions. The calcium-handling proteins sarcalumenin and calsequestrin-1 were increased in control EOM compared with control DIA, reinforcing the view that constitutional properties of EOM are important for their protection against myonecrosis. The finding that galectin-1 (muscle regeneration), annexin A1 (anti-inflammatory) and HSP 47 (fibrosis) were increased in dystrophic diaphragm provides novel insights into the mechanisms through which mdx affected muscles are able to counteract dystrophy, during the early stage of the disease. Overall, the shotgun technique proved to be suitable to perform quantitative comparisons between distinct dystrophic muscles and allowed the suggestion of new potential biomarkers and drug targets for dystrophinopaties.
dc.description8
dc.description6
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionSwedish Research Council
dc.descriptionConselho Nacional de Pesquisas [301306/2010-9]
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionKnut and Alice Wallenberg (KAW) [2007.0118]
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFAPESP [11/51697-6, 08/58491-1, 08/54775-5]
dc.descriptionConselho Nacional de Pesquisas [301306/2010-9]
dc.descriptionCAPES [2014-10-6]
dc.descriptionKnut and Alice Wallenberg (KAW) [2007.0118]
dc.languageen
dc.publisherPublic Library Science
dc.publisherSan Francisco
dc.publisherEUA
dc.relationPlos One
dc.relationPLoS One
dc.rightsaberto
dc.sourceWeb of Science
dc.subjectDuchenne Muscular-dystrophy
dc.subjectMouse Skeletal-muscle
dc.subjectDeficient Muscle
dc.subjectExtracellular-matrix
dc.subjectEndoplasmic-reticulum
dc.subjectShotgun Proteomics
dc.subjectMass-spectrometry
dc.subjectDrastic Increase
dc.subjectAnnexin A1
dc.subjectExpression
dc.titleIsobaric Tagging-Based Quantification for Proteomic Analysis: A Comparative Study of Spared and Affected Muscles from mdx Mice at the Early Phase of Dystrophy
dc.typeArtículos de revistas


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