Aqueous Two-Phase Micellar System for Nisin Extraction in the Presence of Electrolytes

dc.creatorJozala, AF
dc.creatorLopes, AM
dc.creatorNovaes, LCD
dc.creatorMazzola, PG
dc.creatorPenna, TCV
dc.creatorPessoa, A
dc.date2013
dc.dateDEC
dc.date2014-07-30T13:49:27Z
dc.date2015-11-26T16:38:36Z
dc.date2014-07-30T13:49:27Z
dc.date2015-11-26T16:38:36Z
dc.date.accessioned2018-03-28T23:22:00Z
dc.date.available2018-03-28T23:22:00Z
dc.identifierFood And Bioprocess Technology. Springer, v. 6, n. 12, n. 3456, n. 3461, 2013.
dc.identifier1935-5130
dc.identifier1935-5149
dc.identifierWOS:000325869900016
dc.identifier10.1007/s11947-012-1008-1
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/54839
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/54839
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1272296
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionLiquid-liquid extraction for aqueous two-phase micellar systems (ATPMS) is a promising technique that can either replace or be used as a complementary process to more typical chromatographic operations in order to reduce the costs of downstream processing of many biological products. This method offers attractive conditions when exploited in the extraction/purification of a target protein directly on the culture medium. Nisin, an extracellular antimicrobial peptide, is produced by Lactococcus lactis and is effective at controlling a wide range of Gram-positive bacteria, including multidrug-resistant pathogens. This study evaluates ATPMS composed by a nonionic surfactant, Triton X-114, in the presence or absence of electrolytes, to improve the extraction of nisin. The partitioning behavior of nisin showed that it can be directly extracted from the fermentation media. The partitioning coefficient (K (nis)) of the commercial nisin in the presence of electrolytes showed K (nis) values of 5.6 and 5.4 for MgSO4 and (NH4)(2)SO4, respectively. Similar behavior was observed for the produced nisin where K (nis) values were 4.1 and 5.1 for MgSO4 and (NH4)(2)SO4. After partition, the commercial nisin activity in the micelle-rich phase, in the absence of electrolytes, was 3.3 logAU/mL, and in the presence of MgSO4 and (NH4)(2)SO4, the activity was 5.0 logAU/mL. The produced nisin activity with and without MgSO4 was around 3.5 logAU/mL; however, in the presence of (NH4)(2)SO4, nisin activity was 4.5 logAU/mL. The increase in nisin activity after partitioning with salts encourages further researches for the optimization of nisin extraction.
dc.description6
dc.description12
dc.description3456
dc.description3461
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageen
dc.publisherSpringer
dc.publisherNew York
dc.publisherEUA
dc.relationFood And Bioprocess Technology
dc.relationFood Bioprocess Technol.
dc.rightsfechado
dc.rightshttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.sourceWeb of Science
dc.subjectLiquid-liquid extraction
dc.subjectElectrolytes
dc.subjectLactococcus lactis
dc.subjectPeptide purification
dc.subjectNisin
dc.subjectTriton X-114
dc.subjectLiquid-liquid-extraction
dc.subjectLactic-acid Bacteria
dc.subjectLactococcus-lactis
dc.subjectIon-exchange
dc.subjectLps Removal
dc.subjectPurification
dc.subjectSeparation
dc.subjectProteins
dc.subjectSalts
dc.subjectFilms
dc.titleAqueous Two-Phase Micellar System for Nisin Extraction in the Presence of Electrolytes
dc.typeArtículos de revistas


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