Construction and validation of metagenomic DNA libraries from landfarm soil microorganisms

dc.creatorPessoa, TBA
dc.creatorde Souza, SS
dc.creatorCerqueira, AF
dc.creatorRezende, RP
dc.creatorPirovani, CP
dc.creatorDias, JCT
dc.date2013
dc.date2014-07-30T14:02:02Z
dc.date2015-11-26T16:38:34Z
dc.date2014-07-30T14:02:02Z
dc.date2015-11-26T16:38:34Z
dc.date.accessioned2018-03-28T23:21:57Z
dc.date.available2018-03-28T23:21:57Z
dc.identifierGenetics And Molecular Research. Funpec-editora, v. 12, n. 2, n. 2148, n. 2155, 2013.
dc.identifier1676-5680
dc.identifierWOS:000331603100037
dc.identifier10.4238/2013.June.28.2
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/56875
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/56875
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1272284
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionLandfarming biodegradation is a strategy used by the petrochemical industry to reduce pollutants in petroleum-contaminated soil. We constructed 2 metagenomic libraries from landfarming soil in order to determine the pathway used for mineralization of benzene and to examine protein expression of the bacteria in these soils. The DNA of landfarm soil, collected from Ilheus, BA, Brazil, was extracted and a metagenomic library was constructed with the Copy Control (TM) Fosmid Library Production Kit, which clones 25-45-kb DNA fragments. The clones were selected for their ability to express enzymes capable of cleaving aromatic compounds. These clones were grown in Luria-Bertani broth plus L-arabinose, benzene, and chloramphenicol as induction substances; they were tested for activity in the catechol cleavage pathway, an intermediate step in benzene degradation. Nine clones were positive for ortho-cleavage and one was positive for meta-cleavage. Protein band patterns determined by SDS-polyacrylamide gel electrophoresis differed in bacteria grown on induced versus non-induced media (Luria-Bertani broth). We concluded that the DNA of landfarm soil is an important source of genes involved in mineralization of xenobiotic compounds, which are common in gasoline and oil spills. Metagenomic library allows identification of non-culturable microorganisms that have potential in the bioremediation of contaminated sites.
dc.description12
dc.description2
dc.description2148
dc.description2155
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCNPq [558272/2009-6]
dc.languageen
dc.publisherFunpec-editora
dc.publisherRibeirao Preto
dc.publisherBrasil
dc.relationGenetics And Molecular Research
dc.relationGenet. Mol. Res.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectBenzene
dc.subjectToluene
dc.subjectXilene
dc.subjectSoil
dc.subjectMetagenomic library
dc.subjectBiodegradation
dc.subjectPolycyclic Aromatic-hydrocarbons
dc.subjectUncultured Microorganisms
dc.subjectCleavage Pathways
dc.subjectBovine Rumen
dc.subjectDegradation
dc.subjectDiversity
dc.subjectCatechol
dc.subjectBioremediation
dc.subjectDioxygenases
dc.subjectGenes
dc.titleConstruction and validation of metagenomic DNA libraries from landfarm soil microorganisms
dc.typeArtículos de revistas


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