| dc.creator | Silva, ME | |
| dc.creator | Franco, TT | |
| dc.date | 1999 | |
| dc.date | OCT-DEC | |
| dc.date | 2014-12-02T16:28:56Z | |
| dc.date | 2015-11-26T16:38:02Z | |
| dc.date | 2014-12-02T16:28:56Z | |
| dc.date | 2015-11-26T16:38:02Z | |
| dc.date.accessioned | 2018-03-28T23:21:16Z | |
| dc.date.available | 2018-03-28T23:21:16Z | |
| dc.identifier | Revista De Microbiologia. Soc Brasileira Microbiologia, v. 30, n. 4, n. 324, n. 331, 1999. | |
| dc.identifier | 0001-3714 | |
| dc.identifier | WOS:000167874600006 | |
| dc.identifier | http://www.repositorio.unicamp.br/jspui/handle/REPOSIP/58481 | |
| dc.identifier | http://www.repositorio.unicamp.br/handle/REPOSIP/58481 | |
| dc.identifier | http://repositorio.unicamp.br/jspui/handle/REPOSIP/58481 | |
| dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1272189 | |
| dc.description | This work investigated the partitioning of beta -galactosidase from Kluyveromyces fragilis in aqueous two-phase systems (ATPS) by bioaffinity. PEG 4000 was chemically activated with thresyl chloride, and the biospecific ligand p-aminophenyl 1-thio-beta -D-galactopyranoside (APGP) was attached to the activated PEG 4000. A new two-step method for extraction and purification of the enzyme beta -galactosidase from Kluyveromyces fragilis was developed. In the first step, a system composed of 6% PEG 4000-APGP and 8% dextran 505 was used, where beta -galactosidase was strongly partitioned to the top phase (K = 2,330). In the second step, a system formed of 13% PEG-APGP and 9% phosphate salt was used to revert the value of the partition coefficient of beta -galactosidase (K = 2 x 10(-5)) in order to provide the purification and recovery of 39% of the enzyme in the bottom salt-rich phase. | |
| dc.description | 30 | |
| dc.description | 4 | |
| dc.description | 324 | |
| dc.description | 331 | |
| dc.language | en | |
| dc.publisher | Soc Brasileira Microbiologia | |
| dc.publisher | Sao Paulo | |
| dc.publisher | Brasil | |
| dc.relation | Revista De Microbiologia | |
| dc.relation | Rev. Microbiol. | |
| dc.rights | aberto | |
| dc.source | Web of Science | |
| dc.subject | beta-galactosidase | |
| dc.subject | aqueous two-phase systems | |
| dc.subject | protein purification | |
| dc.subject | downstream-processing | |
| dc.subject | affinity | |
| dc.subject | Aqueous 2-phase Systems | |
| dc.subject | Immobilization | |
| dc.subject | Extraction | |
| dc.subject | Proteins | |
| dc.subject | Ligands | |
| dc.subject | Whey | |
| dc.title | Purification of microbial beta-galactosidase from Kluyveromyces fragilis by bioaffinity partitioning | |
| dc.type | Artículos de revistas | |
