Screening, characterization, and biocatalytic capacity of lipases producing wild yeasts from Brazil biomes

dc.creatorGoldbeck, R
dc.creatorMaugeri, F
dc.date2013
dc.dateFEB
dc.date2014-07-30T18:35:45Z
dc.date2015-11-26T16:36:25Z
dc.date2014-07-30T18:35:45Z
dc.date2015-11-26T16:36:25Z
dc.date.accessioned2018-03-28T23:19:09Z
dc.date.available2018-03-28T23:19:09Z
dc.identifierFood Science And Biotechnology. Korean Society Food Science & Technology-kosfost, v. 22, n. 1, n. 79, n. 87, 2013.
dc.identifier1226-7708
dc.identifierWOS:000316017900012
dc.identifier10.1007/s10068-013-0052-6
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/71569
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/71569
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1271808
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionThe main goal of this work was to select and characterize lipase producing wild yeasts isolated from different biomes of Brazil. A total of 372 wild yeasts were tested for their lipase producing potential and 3 strains were selected and characterized according to their substrate specificity, pH, and temperature effects and biocatalytic capacity for synthesis in organic media. For the AC02 strain, the optimal enzymatic conditions were pH 7.0 and temperature 44A degrees C, whilst for AAV1 strain, pH 7.0 was also better, but the optimal temperature was 47A degrees C, and for AY3 strain the optimal temperature was 37A degrees C and pH 6.6. About substrate specificity, the lipase from AY3 strain showed higher specificity for short-chain triglycerides. The AC02 strain showed the highest lipolytic activity among the 3 selected, and was molecularly identified by sequencing the ITS1-5.8S-ITS2 and D1/D2 domains of the subunit (26 S) ribosomal DNA as Metschnikowia pulcherrima.
dc.description22
dc.description1
dc.descriptionS
dc.description79
dc.description87
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageen
dc.publisherKorean Society Food Science & Technology-kosfost
dc.publisherSeoul
dc.publisherCoreia do Sul
dc.relationFood Science And Biotechnology
dc.relationFood Sci. Biotechnol.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectscreening
dc.subjectyeast
dc.subjectlipase
dc.subjectcharacterization
dc.subjectsynthesis organic
dc.subjectExtracellular Lipase
dc.subjectMicrobial Lipases
dc.subjectIdentification
dc.titleScreening, characterization, and biocatalytic capacity of lipases producing wild yeasts from Brazil biomes
dc.typeArtículos de revistas


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