Artículos de revistas
Effects of fungal laccase immobilization procedures for the development of a biosensor for phenol compounds
Registro en:
Talanta. Elsevier Science Bv, v. 54, n. 4, n. 681, n. 686, 2001.
0039-9140
WOS:000169029700015
10.1016/S0039-9140(01)00318-6
Autor
Freire, RS
Duran, N
Kubota, LT
Institución
Resumen
Fungal laccase was immobilized on carbon-fiber electrodes using classical methods: physical adsorption, glutaraldehyde, carbodiimide and carbodiimide/glutaraldehyde. The highest biosensor response was obtained using carbodiimide/glutaraldehyde for coupling laccase to carboxyl groups on the carbon fibers. In this method, different percentages of glutaraldehyde had important effects on the sensitivity of the biosensor, the best percentage of glutaraldehyde being 10% (m/v). The behavior of the obtained biosensor was investigated in terms of sensitivity, operational range, pH and applied potential. The developed biosensor showed an optimum response at pH 5.0 and at an applied potential of - 100 mV. The immobilized laccase retained a good activity for over 2 months. (C) 2001 Elsevier Science B.V. All rights: reserved. 54 4 681 686