Microemulsion extrusion technique: a new method to produce lipid nanoparticles

dc.creatorde Jesus, MB
dc.creatorRadaic, A
dc.creatorZuhorn, IS
dc.creatorde Paula, E
dc.date2013
dc.dateSEP 3
dc.date2014-07-30T14:01:42Z
dc.date2015-11-26T16:35:44Z
dc.date2014-07-30T14:01:42Z
dc.date2015-11-26T16:35:44Z
dc.date.accessioned2018-03-28T23:18:17Z
dc.date.available2018-03-28T23:18:17Z
dc.identifierJournal Of Nanoparticle Research. Springer, v. 15, n. 10, 2013.
dc.identifier1388-0764
dc.identifier1572-896X
dc.identifierWOS:000325251800001
dc.identifier10.1007/s11051-013-1960-3
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/56703
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/56703
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1271605
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionSolid lipid nanoparticles (SLN) and nano-structured lipid carriers (NLC) have been intensively investigated for different applications, including their use as drug and gene delivery systems. Different techniques have been employed to produce lipid nanoparticles, of which high pressure homogenization is the standard technique that is adopted nowadays. Although this method has a high efficiency, does not require the use of organic solvents, and allows large-scale production, some limitations impede its application at laboratory scale: the equipment is expensive, there is a need of huge amounts of surfactants and co-surfactants during the preparation, and the operating conditions are energy intensive. Here, we present the microemulsion extrusion technique as an alternative method to prepare lipid nanoparticles. The parameters to produce lipid nanoparticles using microemulsion extrusion were established, and the lipid particles produced (SLN, NLC, and liposomes) were characterized with regard to size (from 130 to 190 nm), zeta potential, and drug (mitoxantrone) and gene (pDNA) delivery properties. In addition, the particles' in vitro co-delivery capacity (to carry mitoxantrone plus pDNA encoding the phosphatase and tensin homologue, PTEN) was tested in normal (BALB3T3 fibroblast) and cancer (PC3 prostate and MCF-7 breast) cell lines. The results show that the microemulsion extrusion technique is fast, inexpensive, reproducible, free of organic solvents, and suitable for small volume preparations of lipid nanoparticles. Its application is particularly interesting when using rare and/or costly drugs or ingredients (e. g., cationic lipids for gene delivery or labeled lipids for nanoparticle tracking/diagnosis).
dc.description15
dc.description10
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFAPESP [Proc. 2012/01038-9, Proc. 2009/13110-3]
dc.languageen
dc.publisherSpringer
dc.publisherDordrecht
dc.publisherHolanda
dc.relationJournal Of Nanoparticle Research
dc.relationJ. Nanopart. Res.
dc.rightsfechado
dc.rightshttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.sourceWeb of Science
dc.subjectLipid nanoparticles
dc.subjectMicroemulsion extrusion
dc.subjectSolid lipid nanoparticles
dc.subjectNanostructured lipid carrier
dc.subjectLiposomes
dc.subjectOf-the-art
dc.subjectDrug-delivery
dc.subjectBreast-cancer
dc.subjectCo-delivery
dc.subjectResistance
dc.subjectMitoxantrone
dc.subjectSystems
dc.subjectSln
dc.subjectChallenges
dc.subjectTransport
dc.titleMicroemulsion extrusion technique: a new method to produce lipid nanoparticles
dc.typeArtículos de revistas


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