Involvement of Renal Corpuscle microRNA Expression on Epithelial-to-Mesenchymal Transition in Maternal Low Protein Diet in Adult Programmed Rats

dc.creatorSene, LD
dc.creatorMesquita, FF
dc.creatorde Moraes, LN
dc.creatorSantos, DC
dc.creatorCarvalho, R
dc.creatorGontijo, JAR
dc.creatorBoer, PA
dc.date2013
dc.dateAUG 19
dc.date2014-07-30T14:35:18Z
dc.date2015-11-26T16:35:43Z
dc.date2014-07-30T14:35:18Z
dc.date2015-11-26T16:35:43Z
dc.date.accessioned2018-03-28T23:18:16Z
dc.date.available2018-03-28T23:18:16Z
dc.identifierPlos One. Public Library Science, v. 8, n. 8, 2013.
dc.identifier1932-6203
dc.identifierWOS:000323425700037
dc.identifier10.1371/journal.pone.0071310
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/60676
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/60676
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1271602
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionPrior study shows that maternal protein-restricted (LP) 16-wk-old offspring have pronounced reduction of nephron number and arterial hypertension associated with unchanged glomerular filtration rate, besides enhanced glomerular area, which may be related to glomerular hyperfiltration/overflow and which accounts for the glomerular filtration barrier breakdown and early glomerulosclerosis. In the current study, LP rats showed heavy proteinuria associated with podocyte simplification and foot process effacement. TGF-beta 1 glomerular expression was significantly enhanced in LP. Isolated LP glomeruli show a reduced level of miR-200a, miR-141, miR-429 and ZEB2 mRNA and upregulated collagen 1 alpha 1/2 mRNA expression. By western blot analyzes of whole kidney tissue, we found significant reduction of both podocin and nephrin and enhanced expression of mesenchymal protein markers such as desmin, collagen type I and fibronectin. From our present knowledge, these are the first data showing renal miRNA modulation in the protein restriction model of fetal programming. The fetal-programmed adult offspring showed pronounced structural glomerular disorders with an accentuated and advanced stage of fibrosis, which led us to state that the glomerular miR-200 family would be downregulated by TGF-beta 1 action inducing ZEB 2 expression that may subsequently cause glomeruli epithelial-to-mesenchymal transition.
dc.description8
dc.description8
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFAPESP [10/52696-0, 09/54141-9]
dc.languageen
dc.publisherPublic Library Science
dc.publisherSan Francisco
dc.publisherEUA
dc.relationPlos One
dc.relationPLoS One
dc.rightsaberto
dc.sourceWeb of Science
dc.subjectGrowth-factor-beta
dc.subjectMir-200 Family
dc.subjectMessenger-rna
dc.subjectRepressors Zeb1
dc.subjectTargeting Zeb1
dc.subjectBlood-pressure
dc.subjectUnited-states
dc.subjectPodocyte
dc.subjectFibrosis
dc.subjectDisease
dc.titleInvolvement of Renal Corpuscle microRNA Expression on Epithelial-to-Mesenchymal Transition in Maternal Low Protein Diet in Adult Programmed Rats
dc.typeArtículos de revistas


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