Analysis of the biofilm proteome of Xylella fastidiosa

dc.creatorSilva, MS
dc.creatorDe Souza, AA
dc.creatorTakita, MA
dc.creatorLabate, CA
dc.creatorMachado, MA
dc.date2011
dc.dateSEP 22
dc.date2014-07-30T13:49:01Z
dc.date2015-11-26T16:35:20Z
dc.date2014-07-30T13:49:01Z
dc.date2015-11-26T16:35:20Z
dc.date.accessioned2018-03-28T23:17:47Z
dc.date.available2018-03-28T23:17:47Z
dc.identifierProteome Science. Biomed Central Ltd, v. 9, 2011.
dc.identifier1477-5956
dc.identifierWOS:000295597300001
dc.identifier10.1186/1477-5956-9-58
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/54611
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/54611
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1271481
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionBackground: Xylella fastidiosa is limited to the xylem of the plant host and the foregut of insect vectors (sharpshooters). The mechanism of pathogenicity of this bacterium differs from other plant pathogens, since it does not present typical genes that confer specific interactions between plant and pathogens (avr and/or hrp). The bacterium is injected directly into the xylem vessels where it adheres and colonizes. The whole process leads to the formation of biofilms, which are considered the main mechanism of pathogenicity. Cells in biofilms are metabolically and phenotypically different from their planktonic condition. The mature biofilm stage (phase of higher cell density) presents high virulence and resistance to toxic substances such as antibiotics and detergents. Here we performed proteomic analysis of proteins expressed exclusively in the mature biofilm of X. fastidiosa strain 9a5c, in comparison to planktonic growth condition. Results: We found a total of 456 proteins expressed in the biofilm condition, which correspond to approximately 10% of total protein in the genome. The biofilm showed 37% (or 144 proteins) different protein than we found in the planktonic growth condition. The large difference in protein pattern in the biofilm condition may be responsible for the physiological changes of the cells in the biofilm of X. fastidiosa. Mass spectrometry was used to identify these proteins, while real-time quantitative polymerase chain reaction monitored expression of genes encoding them. Most of proteins expressed in the mature biofilm growth were associated with metabolism, adhesion, pathogenicity and stress conditions. Even though the biofilm cells in this work were not submitted to any stress condition, some stress related proteins were expressed only in the biofilm condition, suggesting that the biofilm cells would constitutively express proteins in different adverse environments. Conclusions: We observed overexpression of proteins related to quorum sensing, proving the existence of communication between cells, and thus the development of structuring the biofilm (mature biofilm) leading to obstruction of vessels and development of disease. This paper reports a first proteomic analysis of mature biofilm of X. fastidiosa, opening new perspectives for understanding the biochemistry of mature biofilm growth in a plant pathogen.
dc.description9
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.languageen
dc.publisherBiomed Central Ltd
dc.publisherLondon
dc.publisherInglaterra
dc.relationProteome Science
dc.relationProteome Sci.
dc.rightsaberto
dc.sourceWeb of Science
dc.subjectPseudomonas-aeruginosa
dc.subjectGene-expression
dc.subjectIn-vitro
dc.subjectEscherichia-coli
dc.subjectPathogen
dc.subjectProteins
dc.subjectInvolvement
dc.subjectFimbrial
dc.titleAnalysis of the biofilm proteome of Xylella fastidiosa
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución