Insulin secretion by rat lachrymal glands: effects of systemic and local variables

dc.creatorCunha, DA
dc.creatorCarneiro, EM
dc.creatorAlves, ND
dc.creatorJorge, AG
dc.creatorde Sousa, SM
dc.creatorBoschero, AC
dc.creatorSaad, MJA
dc.creatorVelloso, LA
dc.creatorRocha, EM
dc.date2005
dc.dateNOV
dc.date2014-07-30T14:33:45Z
dc.date2015-11-26T16:35:01Z
dc.date2014-07-30T14:33:45Z
dc.date2015-11-26T16:35:01Z
dc.date.accessioned2018-03-28T23:17:24Z
dc.date.available2018-03-28T23:17:24Z
dc.identifierAmerican Journal Of Physiology-endocrinology And Metabolism. Amer Physiological Soc, v. 289, n. 5, n. E768, n. E775, 2005.
dc.identifier0193-1849
dc.identifierWOS:000232807800005
dc.identifier10.1152/ajpendo.00469.2004
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/60302
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/60302
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1271382
dc.descriptionTo understand the secretory mechanisms and physiological role of insulin in the tear film, the present study examined 1) the time course of insulin secretion in the tear film under glucose intravenous stimulation, 2) the glucose- and carbachol-induced insulin secretion from isolated lachrymal gland (LG), 3) the effect of insulin on glucose consumption by the cornea, and 4) the expression of insulin, pancreatic duodenal homeobox-1 (PDX-1), and glucose transport proteins (GLUTs) in LG tissue. The insulin level in the tear film of 8-wk-old male Wistar rats increased from 0.6 +/- 0.45 to 3.7 +/- 1.3 ng/ml in the initial minutes after glucose stimulation. In vitro assays demonstrated that higher glucose concentrations from 2.8 to 16.7 mM, 200 mu M carbachol, or 40 mM KCl significantly increased insulin secretion from lacrimal glands compared with controls, but did not detect C-peptide as measured by RIA. Glucose consumption by corneal tissue, evaluated by radiolabeled D-[U-C-14] glucose uptake, was 24.07 +/- 0.61 and was enhanced to 31.63 +/- 3.15 nmol center dot cornea(-1) center dot 2 h(-1) in the presence of 6 nM insulin ( P = 0.033) and to 37.5 +/- 3.7 nmol center dot cornea(-1) center dot 2 h(-1) in the presence of 11.2 mM glucose ( P = 0.015). Insulin and PDX-1 mRNA was detected in LG. Insulin was located in the apical areas of acinar cells by immunoperoxidase and the expression of GLUT-1, but not PDX-1, was confirmed by Western blot. These findings suggest that insulin secretion in the tear film is influenced by local stimuli such as nutrient and neural inputs and that this hormone plays a metabolic role in ocular surface tissues. These data also indicate that under normal conditions the insulin secreted by LG is stored, but it is not clear that is locally produced in the LG.
dc.descriptiono TEXTO COMPLETO DESTE ARTIGO, ESTARÁ DISPONÍVEL À PARTIR DE AGOSTO DE 2015.
dc.description289
dc.description5
dc.descriptionE768
dc.descriptionE775
dc.languageen
dc.publisherAmer Physiological Soc
dc.publisherBethesda
dc.publisherEUA
dc.relationAmerican Journal Of Physiology-endocrinology And Metabolism
dc.relationAm. J. Physiol.-Endocrinol. Metab.
dc.rightsembargo
dc.sourceWeb of Science
dc.subjectglucose transportertear filmocular surface
dc.subjectEmbryonic Stem-cells
dc.subjectHuman Ocular Surface
dc.subjectSalivary-glands
dc.subjectImmunoreactive Insulin
dc.subjectGlucose-transporter
dc.subjectDiabetes-mellitus
dc.subjectGrowth-factors
dc.subjectTear Film
dc.subjectIgf-i
dc.subjectEye
dc.titleInsulin secretion by rat lachrymal glands: effects of systemic and local variables
dc.typeArtículos de revistas


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