| dc.creator | Marin, RM | |
| dc.creator | Franchini, KG | |
| dc.creator | Rocco, SA | |
| dc.date | 2007 | |
| dc.date | OCT | |
| dc.date | 2014-07-30T13:48:53Z | |
| dc.date | 2015-11-26T16:34:34Z | |
| dc.date | 2014-07-30T13:48:53Z | |
| dc.date | 2015-11-26T16:34:34Z | |
| dc.date.accessioned | 2018-03-28T23:16:48Z | |
| dc.date.available | 2018-03-28T23:16:48Z | |
| dc.identifier | Journal Of Separation Science. Wiley-v C H Verlag Gmbh, v. 30, n. 15, n. 2473, n. 2479, 2007. | |
| dc.identifier | 1615-9306 | |
| dc.identifier | WOS:000250454100017 | |
| dc.identifier | 10.1002/jssc.200700194 | |
| dc.identifier | http://www.repositorio.unicamp.br/jspui/handle/REPOSIP/54538 | |
| dc.identifier | http://repositorio.unicamp.br/jspui/handle/REPOSIP/54538 | |
| dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1271232 | |
| dc.description | An RP-HPLC method for the analysis of adenosine (ADO) has been developed and validated. In the present study, we report an RP-HPLC-based method with modifications of mobile phase and shorter retention time that substantially improved the efficiency of ADO analysis. The HPLC separation of the ADO was achieved on a C18 column, using a mobile phase consisting of water, containing 7% v/v ACN, at a flow rate of 0.8 mL/min. The column effluent was monitored by UV detection at 260 nm. A linear response was achieved over the concentration range of 0.25-100.00 mu mol/L. The analytical method inter- and intra-run accuracy and precision were better than +/- 15%. The LOQwas 0.25 mu mol/L, with ADO detection in the range of 6.25 pmol per sample. The method has been applied to the study of adenosine kinase (AK) kinetics. | |
| dc.description | 30 | |
| dc.description | 15 | |
| dc.description | 2473 | |
| dc.description | 2479 | |
| dc.language | en | |
| dc.publisher | Wiley-v C H Verlag Gmbh | |
| dc.publisher | Weinheim | |
| dc.publisher | Alemanha | |
| dc.relation | Journal Of Separation Science | |
| dc.relation | J. Sep. Sci. | |
| dc.rights | fechado | |
| dc.rights | http://olabout.wiley.com/WileyCDA/Section/id-406071.html | |
| dc.source | Web of Science | |
| dc.subject | adenosine | |
| dc.subject | adenosine kinase | |
| dc.subject | kinetics | |
| dc.subject | RP-HPLC | |
| dc.subject | Performance Liquid-chromatography | |
| dc.subject | Inhibitors | |
| dc.subject | Heart | |
| dc.subject | Mechanism | |
| dc.subject | Adenine | |
| dc.title | Analysis of adenosine by RP-HPLC method and its application to the study of adenosine kinase kinetics | |
| dc.type | Artículos de revistas | |
