Effects of Preparation Conditions of Poly(lactide-co-glycolide) Nanoparticles Loaded with Amphiphilic Porphyrins and Their Photoactivities

dc.creatorda Silveira, JM
dc.creatorda Silva, AR
dc.creatorSenge, MO
dc.creatorJorge, RA
dc.date2014
dc.dateAUG
dc.date2014-07-30T17:21:43Z
dc.date2015-11-26T16:33:47Z
dc.date2014-07-30T17:21:43Z
dc.date2015-11-26T16:33:47Z
dc.date.accessioned2018-03-28T23:15:48Z
dc.date.available2018-03-28T23:15:48Z
dc.identifierJournal Of Nanoscience And Nanotechnology. Amer Scientific Publishers, v. 14, n. 8, n. 6274, n. 6286, 2014.
dc.identifier1533-4880
dc.identifier1533-4899
dc.identifierWOS:000333579100128
dc.identifier10.1166/jnn.2014.8855
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/65065
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/65065
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1270985
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionThree porphyrins, (5,10,15,20-tetra(3-hydroxyphenyl) porphyrin, 5-hexyl-10,20-bis(3-hydroxyphenyl)porphyrin and 5-hexyl-10,15,20-tris(3-hydroxyphenyl) porphyrin), with different amphiphilicities and equal singlet oxygen quantum yields in ethanol, were encapsulated into 50: 50 poly(lactide-co-glycolide), nanoparticles prepared by the emulsion/evaporation technique. A 2(2) factorial design was utilized to evaluate the influence of the porphyrin/polymer mass ratio and the percentage of ethanol in the aqueous phase on the size and zeta potential of the nanoparticles. Increasing both the amount of ethanol and the porphyrin/polymer ratio decreases the size and increases zeta potential for the photosensitizers studied, except for 5-hexyl-10,15,20-tri(3-hydroxyphenyl) porphyrin. Entrapment efficiency depended on the individual m-hydroxyphenylporphyrin and ranged from 69 to 97%. After 1.5 h incubation with m-hydroxyphenylporphyrin-loaded nanoparticles the percentages of intracellular uptake were the same for all porphyrins since the molecules are confined in the nanoparticles, hampering the interaction of the amphiphilic photosensitizers with the cellular membrane. All encapsulated porphyrins caused the same decrease of cell viability and always localized in the perinuclear region of the cells. Results show that these m-hydroxyphenylporphyrins, although with different amphiphilicities, have equal photodynamic efficacies.
dc.description14
dc.description8
dc.description6274
dc.description6286
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionHealth Research Board
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageen
dc.publisherAmer Scientific Publishers
dc.publisherValencia
dc.publisherEUA
dc.relationJournal Of Nanoscience And Nanotechnology
dc.relationJ. Nanosci. Nanotechnol.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectNanoparticles
dc.subjectFractional Factorial Design
dc.subjectPoly(lactide-co-glycolide)
dc.subjectm-Hydroxyphenylporphyrins
dc.subjectPhotoactivity
dc.subjectPhotodynamic Activity
dc.subjectEmulsification-diffusion
dc.subjectBiodegradable Nanoparticles
dc.subjectSubcellular-localization
dc.subjectPolymeric Nanoparticles
dc.subjectPlga Nanoparticles
dc.subjectCellular Uptake
dc.subjectDrug-delivery
dc.subjectTherapy
dc.subjectTumor
dc.titleEffects of Preparation Conditions of Poly(lactide-co-glycolide) Nanoparticles Loaded with Amphiphilic Porphyrins and Their Photoactivities
dc.typeArtículos de revistas


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