Presence of Spiroplasma penaei in plankton, benthos and fauna in shrimps farms of Colombia

dc.creatorAltamiranda, J
dc.creatorSalazar, M
dc.creatorBrinez, B
dc.date2011
dc.dateMAY-AUG
dc.date2014-07-30T18:08:03Z
dc.date2015-11-26T16:33:18Z
dc.date2014-07-30T18:08:03Z
dc.date2015-11-26T16:33:18Z
dc.date.accessioned2018-03-28T23:15:06Z
dc.date.available2018-03-28T23:15:06Z
dc.identifierRevista Mvz Cordoba. Univ Cordoba, v. 16, n. 2, n. 2576, n. 2583, 2011.
dc.identifier0122-0268
dc.identifierWOS:000297793500016
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/70161
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/70161
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1270804
dc.descriptionObjective. To determine the presence of Spiroplasma penaei on plankton, benhtos and fauna samples, in two commercial shrimp farms. Material and methods. A total of 526 samples of plankton, benthos and fauna were collected; 200 samples were used for histological examination to identify the typical lesions of Spiroplasma, and 326 samples were used for PCR. The samples collected were keep in sterile tubes and preserved in Davidson solution for histological analyses and in 95% ethanol solution for PCR. Results. The presence of Spiroplasma was detected in a water fly through the technique of real time PCR with a melting temperature Tm = 84, similar to the positive control used. This sample was sequenced and compared with sequences of bacteria stored in the GenBank database using the BLAST algorithm, founding 100% homology with a fragment of the 16S ribosomal genes of the bacterium Spiroplasma penaei. Conclusions. The water fly can carry the Spiroplasma penaei, however more experimental assays are required, using water flies infected with Spiroplasma penaei and pathogen-free shrimp to prove this findings.
dc.description16
dc.description2
dc.description2576
dc.description2583
dc.languagees
dc.publisherUniv Cordoba
dc.publisherMonteria
dc.publisherColombia
dc.relationRevista Mvz Cordoba
dc.relationRev. MVZ Cordoba
dc.rightsaberto
dc.sourceWeb of Science
dc.subjectBenhtos
dc.subjectPCR
dc.subjectPenaeus vannamei
dc.subjectplankton
dc.subjectSpiroplasma
dc.subjectChinese Mitten Crab
dc.subjectPolymerase-chain-reaction
dc.subjectPacific White Shrimp
dc.subjectEriocheir-sinensis
dc.subjectTremor Disease
dc.subjectVannamei
dc.subjectManagement
dc.subjectAlignment
dc.subjectCrayfish
dc.titlePresence of Spiroplasma penaei in plankton, benthos and fauna in shrimps farms of Colombia
dc.typeArtículos de revistas


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