Investigation of mutations associated with pyrethroid resistance in populations of the New World Screwworm fly, Cochliomyia hominivorax (Diptera: Calliphoridae)

dc.creatorda Silva, NM
dc.creatorde Azeredo-Espin, AML
dc.date2009
dc.date2014-11-17T03:31:24Z
dc.date2015-11-26T16:33:13Z
dc.date2014-11-17T03:31:24Z
dc.date2015-11-26T16:33:13Z
dc.date.accessioned2018-03-28T23:14:59Z
dc.date.available2018-03-28T23:14:59Z
dc.identifierGenetics And Molecular Research. Funpec-editora, v. 8, n. 3, n. 1067, n. 1078, 2009.
dc.identifier1676-5680
dc.identifierWOS:000272050000014
dc.identifier10.4238/vol8-3gmr643
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/60621
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/60621
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/60621
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1270773
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCochliomyia hominivorax larvae are known for their parasitic habit in living vertebrates, causing considerable economic losses to livestock industry. This ectoparasite has been controlled mainly by applying insecticides, but this method usually results in the selection of resistant individuals. The resistance mechanism known as knockdown resistance (kdr) is a generic term for amino acid substitutions in the sodium channel associated with pyrethroid resistance, and substitutions in residue 251 of the carboxylesterase E3 have been associated with organophosphate and pyrethroid hydrolysis. We looked for L1014F kdr and W251S mutations in the sodium channel and E3 genes, respectively, in C. hominivorax populations. Ten populations obtained from its current distribution were investigated using the polymerase chain reaction-restriction fragment length polymorphism technique. No mutant individuals were found for the kdr mutation. However, the W251S mutation was found in all populations investigated. Results from a bioassay with cypermethrin (a pyrethroid) indicated that the survival at the lowest concentration (Fisher exact test, P = 0.0003) and an intermediate concentration (P = 0.024) were associated with the W251S mutation. This correlation was not significant at the highest concentration tested (P = 0.221). We suggest that the W251S mutation in the C. hominivorax carboxylesterase E3 is also associated with pyrethroid hydrolysis. This information may contribute to the elaboration of improved management programs for this ectoparasite.
dc.description8
dc.description3
dc.description1067
dc.description1078
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFAPESP [03/01458-9, 07/54431-1]
dc.descriptionCNPq [141277/2005-0]
dc.languageen
dc.publisherFunpec-editora
dc.publisherRibeirao Preto
dc.publisherBrasil
dc.relationGenetics And Molecular Research
dc.relationGenet. Mol. Res.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectCochliomyia hominivorax
dc.subjectInsecticide resistance
dc.subjectkdr
dc.subjectCarboxylesterase
dc.subjectOrganophosphorus Insecticide Resistance
dc.subjectLucilia-cuprina
dc.subjectKnockdown Resistance
dc.subjectCross-resistance
dc.subjectEsterase
dc.subjectCarboxylesterases
dc.subjectIdentification
dc.subjectKdr
dc.subjectE3
dc.subjectEradication
dc.titleInvestigation of mutations associated with pyrethroid resistance in populations of the New World Screwworm fly, Cochliomyia hominivorax (Diptera: Calliphoridae)
dc.typeArtículos de revistas


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