COMPARISON OF INDIRECT IMMUNOFLUORESCENCE WITH AGAR-GEL IMMUNODIFFUSION FOR THE DIAGNOSIS OF BLUETONGUE VIRUS-INFECTION

dc.creatorARITA, GMM
dc.creatorGATTI, MSV
dc.creatorGERMANO, PML
dc.creatorPESTANADECASTRO, AF
dc.date1992
dc.date2014-07-30T14:00:12Z
dc.date2015-11-26T16:32:56Z
dc.date2014-07-30T14:00:12Z
dc.date2015-11-26T16:32:56Z
dc.date.accessioned2018-03-28T23:14:33Z
dc.date.available2018-03-28T23:14:33Z
dc.identifierBrazilian Journal Of Medical And Biological Research. Assoc Bras Divulg Cientifica, v. 25, n. 5, n. 503, n. 508, 1992.
dc.identifier0100-879X
dc.identifierWOS:A1992HY84800010
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/56256
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/56256
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1270689
dc.description1. Sera from 190 cows and from 72 sheep were examined to compare the results obtained with the agar gel immunodiffusion (AGID) and indirect immunofluorescence (IIF) tests for the diagnosis of bluetongue (BT) disease. 2. In the AGID test, 96 of 190 (50.5%) cattle serum samples and 38 of 72 (52.7%) sheep serum samples were positive, for a total of 134 out of 262 (51.1%) sera. In the IIF test, 98 of 190 (51.6%) cattle serum samples and 39 of 72 (54.2%) sheep serum samples were positive, for a total of 137 out of 262 (52.3%) sera. 3. The fluorescence of the IIF test presented a granular cytoplasmic aspect, which in some cells was observed only on the cell membranes. 4. Statistical analysis of the data showed close agreement between the two techniques, regardless of the kind of sera examined. The IIF test showed high sensitivity (93.8% and 92.1%), specificity (91.4% and 88.2%) and positive (91.8% and 89.7%) and negative (93.48% and 90.9%) predictive values for cattle serum and sheep serum, respectively. 5. The results obtained with IIF were comparable to those obtained with the AGID test, indicating that both techniques can be used routinely in epidemiologic studies of BT. However, the IIF offers the additional advantages that it can be used for antibody quantification and for the detection of viral antigens in BT-infected cell lines.
dc.description25
dc.description5
dc.description503
dc.description508
dc.languageen
dc.publisherAssoc Bras Divulg Cientifica
dc.publisherSao Paulo
dc.publisherBrasil
dc.relationBrazilian Journal Of Medical And Biological Research
dc.relationBrazilian J. Med. Biol. Res.
dc.rightsaberto
dc.sourceWeb of Science
dc.subjectBLUETONGUE VIRUS
dc.subjectIMMUNODIFFUSION TEST
dc.subjectINDIRECT IMMUNOFLUORESCENCE
dc.titleCOMPARISON OF INDIRECT IMMUNOFLUORESCENCE WITH AGAR-GEL IMMUNODIFFUSION FOR THE DIAGNOSIS OF BLUETONGUE VIRUS-INFECTION
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución