Comparison of Endotoxin Levels Found in Primary and Secondary Endodontic Infections

dc.creatorGomes, BPFA
dc.creatorEndo, MS
dc.creatorMartinho, FC
dc.date2012
dc.dateAUG
dc.date2014-07-30T14:00:10Z
dc.date2015-11-26T16:32:54Z
dc.date2014-07-30T14:00:10Z
dc.date2015-11-26T16:32:54Z
dc.date.accessioned2018-03-28T23:14:31Z
dc.date.available2018-03-28T23:14:31Z
dc.identifierJournal Of Endodontics. Elsevier Science Inc, v. 38, n. 8, n. 1082, n. 1086, 2012.
dc.identifier0099-2399
dc.identifierWOS:000307028100009
dc.identifier10.1016/j.joen.2012.04.021
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/56243
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/56243
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1270680
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionIntroduction: This clinical study was conducted to compare the levels of endotoxins (lipopolysaccharides [LPSs]) found in primary and secondary endodontic infections with apical periodontitis by correlating LPS contents with clinical/radiographic findings. In addition, the presence of target gram-negative anaerobic bacteria was also investigated. Methods: Samples were taken from 15 root canals with primary infections and 15 with secondary infections by using paper points. The limulus amebocyte lysate assay was used to quantify endotoxins, and the polymerase chain reaction technique (16S rDNA) was used for bacterial investigation. Results: Endotoxins were detected in 100% of the root canal samples collected from primary (15/15) and secondary (15/15) infections with median values of 7.49 EU/mL and 3.96 EU/mL, respectively (P < .05). The median value of endotoxins found in the presence of clinical symptoms was significantly higher than in asymptomatic teeth with primary infections (P < .05). A positive correlation was found between endotoxin contents and a larger size of the radiolucent area (> 3 mm) (P < .05). Prevotella nigrescens (10/15, 4/15), Fusobacterium nucleatum (5/15, 1/15), Treponema denticola (3/15, 1/15), and Treponema socranskii (5/15, 1/15) were detected in teeth with primary and secondary infections, respectively. P endodontalis was present only in teeth with primary infections (5/15). Conclusions: Teeth with primary endodontic infections had higher contents of endotoxins and a more complex gram-negative bacterial community than teeth with secondary infections. Moreover, the levels of endotoxins were related to the severity of bone destruction in periapical tissues as well as the development of clinical features in teeth with primary infections. (J Endod 2012;38:1082-1086)
dc.description38
dc.description8
dc.description1082
dc.description1086
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFAPESP [08/57954-8, 08/57551-0, 10/19136-1, 10/17877-4, 11/50051-5, 11/50510-0]
dc.descriptionCNPq [302575/2009-0, 150557/2011-6]
dc.languageen
dc.publisherElsevier Science Inc
dc.publisherNew York
dc.publisherEUA
dc.relationJournal Of Endodontics
dc.relationJ. Endod.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectEndodontic infection
dc.subjectendotoxin
dc.subjectlimulus amebocyte lysate assay
dc.subjectroot canal
dc.subjectPolymerase-chain-reaction
dc.subjectRoot-canal Infection
dc.subjectPeriapical Lesions
dc.subjectChemomechanical Preparation
dc.subjectMolecular Analysis
dc.subjectImmune-response
dc.subjectFilled Teeth
dc.subjectLipopolysaccharide
dc.subjectBacteroides
dc.subjectQuantification
dc.titleComparison of Endotoxin Levels Found in Primary and Secondary Endodontic Infections
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución