Cloning, Characterization and Analysis of cat and ben Genes from the Phenol Degrading Halophilic Bacterium Halomonas organivorans

dc.creatorMoreno, MD
dc.creatorSanchez-Porro, C
dc.creatorPiubeli, F
dc.creatorFrias, L
dc.creatorGarcia, MT
dc.creatorMellado, E
dc.date2011
dc.date40330
dc.date2014-07-30T13:59:55Z
dc.date2015-11-26T16:32:41Z
dc.date2014-07-30T13:59:55Z
dc.date2015-11-26T16:32:41Z
dc.date.accessioned2018-03-28T23:14:11Z
dc.date.available2018-03-28T23:14:11Z
dc.identifierPlos One. Public Library Science, v. 6, n. 6, 2011.
dc.identifier1932-6203
dc.identifierWOS:000291612600043
dc.identifier10.1371/journal.pone.0021049
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/56107
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/56107
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1270598
dc.descriptionBackground: Extensive use of phenolic compounds in industry has resulted in the generation of saline wastewaters that produce significant environmental contamination; however, little information is available on the degradation of phenolic compounds in saline conditions. Halomonas organivorans G-16.1 (CECT 5995(T)) is a moderately halophilic bacterium that we isolated in a previous work from saline environments of South Spain by enrichment for growth in different pollutants, including phenolic compounds. PCR amplification with degenerate primers revealed the presence of genes encoding ring-cleaving enzymes of the beta-ketoadipate pathway for aromatic catabolism in H. organivorans. Findings: The gene cluster catRBCA, involved in catechol degradation, was isolated from H. organivorans. The genes catA, catB, catC and the divergently transcribed catR code for catechol 1,2-dioxygenase (1,2-CTD), cis, cis-muconate cycloisomerase, muconolactone delta-isomerase and a LysR-type transcriptional regulator, respectively. The benzoate catabolic genes (benA and benB) are located flanking the cat genes. The expression of cat and ben genes by phenol and benzoic acid was shown by RT-PCR analysis. The induction of catA gene by phenol and benzoic acid was also probed by the measurement of 1,2-CTD activity in H. organivorans growth in presence of these inducers. 16S rRNA and catA gene-based phylogenies were established among different degrading bacteria showing no phylogenetic correlation between both genes. Conclusions/Significance: In this work, we isolated and determined the sequence of a gene cluster from a moderately halophilic bacterium encoding ortho-pathway genes involved in the catabolic metabolism of phenol and analyzed the gene organization, constituting the first report characterizing catabolic genes involved in the degradation of phenol in moderate halophiles, providing an ideal model system to investigate the potential use of this group of extremophiles in the decontamination of saline environments.
dc.description6
dc.description6
dc.descriptionSpanish Ministry of Science and Education [CTM2006-03310, BIO2006-06927]
dc.descriptionJunta de Andalucia [P08-RNM-3515]
dc.descriptionSpanish Ministry of Science and Education [CTM2006-03310, BIO2006-06927]
dc.descriptionJunta de Andalucia [P08-RNM-3515]
dc.languageen
dc.publisherPublic Library Science
dc.publisherSan Francisco
dc.publisherEUA
dc.relationPlos One
dc.relationPLoS One
dc.rightsaberto
dc.sourceWeb of Science
dc.subjectCatechol 1,2-dioxygenase
dc.subjectPseudomonas-putida
dc.subjectWaste-water
dc.subjectDegradation
dc.subjectBiodegradation
dc.subjectStrain
dc.subjectBioremediation
dc.subjectEnvironments
dc.subjectCatabolism
dc.subjectPathways
dc.titleCloning, Characterization and Analysis of cat and ben Genes from the Phenol Degrading Halophilic Bacterium Halomonas organivorans
dc.typeArtículos de revistas


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