Assessment of the Myogenic Stem Cell Compartment Following Transplantation of Pax3/Pax7-Induced Embryonic Stem Cell-Derived Progenitors

dc.creatorDarabi, R
dc.creatorSantos, FNC
dc.creatorFilareto, A
dc.creatorPan, WH
dc.creatorKoene, R
dc.creatorRudnicki, MA
dc.creatorKyba, M
dc.creatorPerlingeiro, RCR
dc.date2011
dc.dateMAY
dc.date2014-07-30T13:51:10Z
dc.date2015-11-26T16:32:34Z
dc.date2014-07-30T13:51:10Z
dc.date2015-11-26T16:32:34Z
dc.date.accessioned2018-03-28T23:14:02Z
dc.date.available2018-03-28T23:14:02Z
dc.identifierStem Cells. Wiley-blackwell, v. 29, n. 5, n. 777, n. 790, 2011.
dc.identifier1066-5099
dc.identifierWOS:000289719200006
dc.identifier10.1002/stem.625
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/55014
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/55014
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1270557
dc.descriptionAn effective long-term cell therapy for skeletal muscle regeneration requires donor contribution to both muscle fibers and the muscle stem cell pool. Although satellite cells have these abilities, their therapeutic potential so far has been limited due to their scarcity in adult muscle. Myogenic progenitors obtained from Pax3-engineered mouse embryonic stem (ES) cells have the ability to generate myofibers and to improve the contractility of transplanted muscles in vivo, however, whether these cells contribute to the muscle stem cell pool and are able to self-renew in vivo are still unknown. Here, we addressed this question by investigating the ability of Pax3, which plays a critical role in embryonic muscle formation, and Pax7, which is important for maintenance of the muscle satellite cell pool, to promote the derivation of self-renewing functional myogenic progenitors from ES cells. We show that Pax7, like Pax3, can drive the expansion of an ES-derived myogenic progenitor with significant muscle regenerative potential. We further demonstrate that a fraction of transplanted cells remains mononuclear, and displays key features of skeletal muscle stem cells, including satellite cell localization, response to reinjury, and contribution to muscle regeneration in secondary transplantation assays. The ability to engraft, self-renew, and respond to injury provide foundation for the future therapeutic application of ES-derived myogenic progenitors in muscle disorders. STEM CELLS 2011;29:777-790
dc.description29
dc.description5
dc.description777
dc.description790
dc.descriptionDr. Bob and Jean Smith Foundation
dc.descriptionNIAMS [AR055299]
dc.descriptionNIA at the National Institutes of Health [AG034370]
dc.descriptionNIAMS [AR055299]
dc.descriptionNIA at the National Institutes of Health [AG034370]
dc.languageen
dc.publisherWiley-blackwell
dc.publisherMalden
dc.publisherEUA
dc.relationStem Cells
dc.relationStem Cells
dc.rightsfechado
dc.rightshttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.sourceWeb of Science
dc.subjectES cells
dc.subjectPax7/Pax3
dc.subjectSatellite cells
dc.subjectSelf-renewal
dc.subjectSkeletal muscle progenitors
dc.subjectMuscle Satellite Cells
dc.subjectSelf-renewal
dc.subjectGene-expression
dc.subjectHematopoietic Progenitors
dc.subjectRegeneration
dc.subjectPax-3
dc.subjectMice
dc.subjectSpecification
dc.subjectEngraftment
dc.subjectFibers
dc.titleAssessment of the Myogenic Stem Cell Compartment Following Transplantation of Pax3/Pax7-Induced Embryonic Stem Cell-Derived Progenitors
dc.typeArtículos de revistas


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