Local injection of BDNF producing mesenchymal stem cells increases neuronal survival and synaptic stability following ventral root avulsion

dc.creatorHell, RCR
dc.creatorCosta, MMS
dc.creatorGoes, AM
dc.creatorOliveira, ALR
dc.date2009
dc.dateFEB
dc.date2014-11-16T01:11:45Z
dc.date2015-11-26T16:31:20Z
dc.date2014-11-16T01:11:45Z
dc.date2015-11-26T16:31:20Z
dc.date.accessioned2018-03-28T23:12:22Z
dc.date.available2018-03-28T23:12:22Z
dc.identifierNeurobiology Of Disease. Academic Press Inc Elsevier Science, v. 33, n. 2, n. 290, n. 300, 2009.
dc.identifier0969-9961
dc.identifierWOS:000263120500017
dc.identifier10.1016/j.nbd.2008.10.017
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/52547
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/52547
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/52547
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1270177
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionThe present study proposed to graft mesenchymal stem cells (MSCs), which continuously produce BDNF, into the spinal cord ventral horn, after ventral root avulsion. Neurotrophin expression was naturally achieved by culturing MSCs in an undifferentiated state for at least 10 weeks. Lewis rats were subjected to cells injected through the lateral funiculus. unilateral avulsion of lumbar ventral roots, receiving 3 x 10(5) Two weeks after surgery, the animals were sacrificed and neuronal survival, astroglial reaction and synaptic inputs within the motor nucleus analyzed. The results indicated that the MSCs treatment significantly rescued avulsed motoneurons. Such neuronal survival was related to in vivo mRNA up regulation as well as expression of BDNF and GDNF. Such increase was correlated to the preservation of synaptophysin- positive nerve terminals. Thus it was proposed that when maintained undifferentiated for a period of 10 weeks, MSCs may be used as a continuous source of BDNF, positively influencing neuronal survival and synaptic plasticity. (C) 2008 Elsevier Inc. All rights reserved.
dc.description33
dc.description2
dc.description290
dc.description300
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCNPq [24/2005]
dc.languageen
dc.publisherAcademic Press Inc Elsevier Science
dc.publisherSan Diego
dc.publisherEUA
dc.relationNeurobiology Of Disease
dc.relationNeurobiol. Dis.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectStem cells
dc.subjectMotoneuron
dc.subjectSpinal cord
dc.subjectVentral root avulsion
dc.subjectGlial reaction
dc.subjectMarrow Stromal Cells
dc.subjectAmyotrophic-lateral-sclerosis
dc.subjectSpinal-cord-injury
dc.subjectMessenger-rna Expression
dc.subjectGrowth-factor Production
dc.subjectTraumatic Brain-injury
dc.subjectNeurotrophic Factor
dc.subjectAdult-rat
dc.subjectAxonal Regeneration
dc.subjectFunctional Recovery
dc.titleLocal injection of BDNF producing mesenchymal stem cells increases neuronal survival and synaptic stability following ventral root avulsion
dc.typeArtículos de revistas


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