Ibuprofen-induced Walker 256 tumor cell death: cytochrome c release from functional mitochondria and enhancement by calcineurin inhibition

dc.creatorCampos, CBL
dc.creatorDegasperi, GR
dc.creatorPacifico, DS
dc.creatorAlberici, LC
dc.creatorCarreira, RS
dc.creatorGuimaraes, F
dc.creatorCastilho, RF
dc.creatorVercesi, AE
dc.date2004
dc.dateDEC 1
dc.date2014-11-15T03:00:35Z
dc.date2015-11-26T16:30:33Z
dc.date2014-11-15T03:00:35Z
dc.date2015-11-26T16:30:33Z
dc.date.accessioned2018-03-28T23:11:36Z
dc.date.available2018-03-28T23:11:36Z
dc.identifierBiochemical Pharmacology. Pergamon-elsevier Science Ltd, v. 68, n. 11, n. 2197, n. 2206, 2004.
dc.identifier0006-2952
dc.identifierWOS:000224952200011
dc.identifier10.1016/j.bcp.2004.08.006
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/69097
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/69097
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/69097
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1269988
dc.descriptionThe participation of mitochondria in the mechanism of tumor cell death induced by non-steroid anti-inflammatory drugs is uncertain. Here we show that ibuprofen induces death of Walker 256 tumor cells independently on mitochondrial depolarization as estimated by flow cytometry using DiOC(6)(3). Oligomycin increased mitochondrial transmembrane potential in both ibuprofen-treated and non-treated cells, indicating that ATP synthesis was sustained during cell death. Cyclosporin A, but not bongkrekic acid, both mitochondrial permeability transition inhibitors, increased the percentage of cell death in the presence of ibuprofen. FK506, a calcineurin inhibitor like cyclosporin A, also increased ibuprofen-induced cell death. Moreover, we showed that cytochrome c was released during ibuprofen-induced cell death. In conclusion, death of Walker 256 tumor cells induced by ibuprofen does not impair mitochondrial function, involves cytochrome c release and is accompanied by a rescue pathway via calcineurin activation. (C) 2004 Elsevier Inc. All rights reserved.
dc.description68
dc.description11
dc.description2197
dc.description2206
dc.languageen
dc.publisherPergamon-elsevier Science Ltd
dc.publisherOxford
dc.publisherInglaterra
dc.relationBiochemical Pharmacology
dc.relationBiochem. Pharmacol.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectapoptosis
dc.subjectibuprofen
dc.subjectmitochondrion
dc.subjectelectrical transmembrane potential
dc.subjectcytochrome c
dc.subjectcalcineurin
dc.subjectNonsteroidal Antiinflammatory Drugs
dc.subjectProstate-cancer Cells
dc.subjectPermeability Transition
dc.subjectInduced Apoptosis
dc.subjectMembrane Permeabilization
dc.subjectCyclooxygenase-2 Inhibitor
dc.subjectCis/trans Isomerases
dc.subjectOxidative Stress
dc.subjectFlow-cytometry
dc.subjectCyclosporine-a
dc.titleIbuprofen-induced Walker 256 tumor cell death: cytochrome c release from functional mitochondria and enhancement by calcineurin inhibition
dc.typeArtículos de revistas


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