Iron(III) tetra-(N-methyl-4-pyridyl)-porphyrin as a biomimetic catalyst of horseradish peroxidase on the electrode surface: An amperometric sensor for phenolic compound determinations

dc.creatorDamos, FS
dc.creatorSotomayor, MDT
dc.creatorKubota, LT
dc.creatorTanaka, SMCN
dc.creatorTanaka, AA
dc.date2003
dc.date2014-11-19T21:23:48Z
dc.date2015-11-26T16:29:42Z
dc.date2014-11-19T21:23:48Z
dc.date2015-11-26T16:29:42Z
dc.date.accessioned2018-03-28T23:10:46Z
dc.date.available2018-03-28T23:10:46Z
dc.identifierAnalyst. Royal Soc Chemistry, v. 128, n. 3, n. 255, n. 259, 2003.
dc.identifier0003-2654
dc.identifierWOS:000181357900010
dc.identifier10.1039/b207894e
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/60730
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/60730
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/60730
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1269793
dc.descriptionThe use of iron(III) tetra-(N-methyl-4-pyridyl) porphyrin (Fe(III)T4MpyP) and histidine (His) in the construction of an amperometric sensor for phenolic compound determinations is reported, based on horseradish peroxidase (HRP) chemistry. The sensor was prepared by modifying a glassy carbon electrode with Nafion(R) membrane doped with Fe-III T4MpyP and His, in a mass ratio of 1:2. The sensor presented its best performance at 50 mV vs. SCE in 0.1 mol l(-1) succinate buffer (pH = 4.0) containing 125 mumol l(-1) H2O2. Under optimized operational conditions, a linear response range from 0.6 to 6.0 mumol l(-1) was obtained with a sensitivity of 61 nA cm(-2) mumol l(-1). The detection limit for catechol determination was 0.35 mumol l(-1). The response time was less than 0.5 s. The proposed sensor presented stable responses for 100 successive determinations, while satisfactory responses were observed even after 200 measurements. The repeatability, evaluated in terms of relative standard deviation, was 4% for n = 7. The signal responses for other phenolic compounds, including those of environmental and clinical interest, were also investigated.
dc.description128
dc.description3
dc.description255
dc.description259
dc.languageen
dc.publisherRoyal Soc Chemistry
dc.publisherCambridge
dc.publisherInglaterra
dc.relationAnalyst
dc.relationAnalyst
dc.rightsaberto
dc.sourceWeb of Science
dc.subjectHydrogen-peroxide
dc.subjectGraphite-electrodes
dc.subjectSol-gel
dc.subjectBiosensor
dc.subjectPorphyrin
dc.subjectSubstrate
dc.subjectEnzymes
dc.subjectMechanism
dc.subjectAssay
dc.subjectModel
dc.titleIron(III) tetra-(N-methyl-4-pyridyl)-porphyrin as a biomimetic catalyst of horseradish peroxidase on the electrode surface: An amperometric sensor for phenolic compound determinations
dc.typeArtículos de revistas


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