| dc.creator | Fernandes, JR | |
| dc.creator | Neto, GD | |
| dc.creator | Tubino, M | |
| dc.creator | Kubota, LT | |
| dc.date | 1996 | |
| dc.date | JUN | |
| dc.date | 2014-12-16T11:35:36Z | |
| dc.date | 2015-11-26T16:29:00Z | |
| dc.date | 2014-12-16T11:35:36Z | |
| dc.date | 2015-11-26T16:29:00Z | |
| dc.date.accessioned | 2018-03-28T23:10:04Z | |
| dc.date.available | 2018-03-28T23:10:04Z | |
| dc.identifier | Analytical Sciences. Japan Soc Analytical Chem, v. 12, n. 3, n. 443, n. 447, 1996. | |
| dc.identifier | 0910-6340 | |
| dc.identifier | WOS:A1996UQ75900011 | |
| dc.identifier | 10.2116/analsci.12.443 | |
| dc.identifier | http://www.repositorio.unicamp.br/jspui/handle/REPOSIP/79562 | |
| dc.identifier | http://www.repositorio.unicamp.br/handle/REPOSIP/79562 | |
| dc.identifier | http://repositorio.unicamp.br/jspui/handle/REPOSIP/79562 | |
| dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1269619 | |
| dc.description | A new methodology for soluble oxalic acid determination in grass samples was developed using a two enzyme reactor in an FIA system. The reactor consisted of 3 U of oxalate oxidase and 100 U of peroxidase immobilized on Sorghum vulgare seeds activated with glutaraldehyde. The carbon dioxide was monitored spectrophotometrically, after reacting with an acid-base indicator (Bromocresol Purple) after it permeated through a PTFE membrane. A linear response range was observed between 0.25 and 1.00 mmol l(-1) of oxalic acid; the data was fit by the equation A = -0.8(+/-1.5)+57.2(+/-2.5)[oxalate], with a correlation coefficient of 0.9971 and a relative standard deviation of 2% for n=5. The variance for a 0.25 mmol l(-1) oxalic acid standard solution was lower than 4% for 11 measurements. The FIA system allows analysis of 20 samples per hour without prior treatment. The proposed method showed a good correlation with that of the Sigma Kit. | |
| dc.description | 12 | |
| dc.description | 3 | |
| dc.description | 443 | |
| dc.description | 447 | |
| dc.language | en | |
| dc.publisher | Japan Soc Analytical Chem | |
| dc.publisher | Tokyo | |
| dc.publisher | Japão | |
| dc.relation | Analytical Sciences | |
| dc.relation | Anal. Sci. | |
| dc.rights | fechado | |
| dc.source | Web of Science | |
| dc.subject | oxalate determination | |
| dc.subject | oxalate oxidase | |
| dc.subject | grass | |
| dc.subject | Sorghum vulgare seeds | |
| dc.subject | Flow-injection Determination | |
| dc.subject | Urine Oxalate | |
| dc.title | Determination of oxalate in grass by FIA with a spectrophotometric detection system using a two enzyme reactor | |
| dc.type | Artículos de revistas | |
