Reversion of hepatic steatosis by exercise training in obese mice: The role of sterol regulatory element-binding protein-1c

dc.creatorCintra, DE
dc.creatorRopelle, ER
dc.creatorVitto, MF
dc.creatorLuciano, TF
dc.creatorSouza, DR
dc.creatorEngelmann, J
dc.creatorMarques, SO
dc.creatorLira, FS
dc.creatorde Pinho, RA
dc.creatorPauli, JR
dc.creatorDe Souza, CT
dc.date2012
dc.dateOCT 5
dc.date2014-08-01T18:29:58Z
dc.date2015-11-26T16:28:48Z
dc.date2014-08-01T18:29:58Z
dc.date2015-11-26T16:28:48Z
dc.date.accessioned2018-03-28T23:09:51Z
dc.date.available2018-03-28T23:09:51Z
dc.identifierLife Sciences. Pergamon-elsevier Science Ltd, v. 91, n. 41984, n. 395, n. 401, 2012.
dc.identifier0024-3205
dc.identifierWOS:000309645100006
dc.identifier10.1016/j.lfs.2012.08.002
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/79789
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/79789
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1269565
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionAim: The dysregulation of regulatory element-binding protein-1c (SREBP-1c) is associated with hepatic steatosis. However, effects of exercise on SREBP-1c protein level in liver have not been investigated. Thus, in this study we investigated if reversion of the hepatic steatosis-induced by exercise training is related with levels of SREBP-1c. Main methods: Mice were divided into two groups: control lean mice (CT), fed on standard rodent chow, and obese mice (HF), fed on a high-fat diet for 2 months. After this period obese mice were divided in two groups: obese mice and obese mice submitted to exercise (HF + EXE). The HF + EXE group performed a running program of 50 min per day, 5 days per week, for 8 weeks. Forty-eight hours after the last exercise session, biochemical, immunoblotting, histology and immunohistochemistry analyses were performed. Key findings: Livers of HF mice showed increased SREBP-1c, FAS (Fatty Acid Synthase), SCD1 (Stearoyl-CoA Desaturase1) and CPT1 (Carnitine Palmitoyl Transferase1) protein levels (3.4, 5.0, 2.6 and 2.9 times, respectively), though ACC (Acetyl-CoA Carboxilase) phosphorylation dropped 4.2 times. In livers of HF + EXE, levels of SREBP-1c, FAS, SCDI and CPTI decreased 2.1, 1.9, 1.8, and 2.7 times, respectively), while ACC phosphorylation increased 3.0 times. Lower SREBP-1c protein levels after exercise were confirmed also by immunohistochemistry. Total liver lipids content was higher in HF (2.2 times) when compared to CT, and exercise training reduced it significantly (1.7 times). Significance: Our study allows concluding that the reduction in SREBP-1c protein levels is associated with steatosis reversion induced by exercise training. Crown Copyright (c) 2012 Published by Elsevier Inc. All rights reserved.
dc.description91
dc.description41984
dc.description395
dc.description401
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionUniversity Southern of Santa Catarina (UNESC)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageen
dc.publisherPergamon-elsevier Science Ltd
dc.publisherOxford
dc.publisherInglaterra
dc.relationLife Sciences
dc.relationLife Sci.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectSterol regulatory element-binding protein-1c
dc.subjectExercise training
dc.subjectHepatic steatosis
dc.subjectFatty Liver-disease
dc.subjectStearoyl-coa Desaturase-1
dc.subjectShort-term Inhibition
dc.subjectInsulin-resistance
dc.subjectSkeletal-muscle
dc.subjectWeight-loss
dc.subjectExpression
dc.subjectRats
dc.subjectDeficiency
dc.subjectMetabolism
dc.titleReversion of hepatic steatosis by exercise training in obese mice: The role of sterol regulatory element-binding protein-1c
dc.typeArtículos de revistas


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