Comparative evaluation of conventional RT-PCR and real-time RT-PCR (RRT-PCR) for detection of avian metapneumovirus subtype A

dc.creatorFerreira, HL
dc.creatorSpilki, FR
dc.creatordos Santos, MMAB
dc.creatorde Almeida, RS
dc.creatorArns, CW
dc.date2009
dc.dateAUG
dc.date2014-08-01T18:27:19Z
dc.date2015-11-26T16:28:18Z
dc.date2014-08-01T18:27:19Z
dc.date2015-11-26T16:28:18Z
dc.date.accessioned2018-03-28T23:09:18Z
dc.date.available2018-03-28T23:09:18Z
dc.identifierCiencia Rural. Univ Federal Santa Maria, v. 39, n. 5, n. 1445, n. 1451, 2009.
dc.identifier0103-8478
dc.identifierWOS:000268847700022
dc.identifier10.1590/S0103-84782009005000057
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/79146
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/79146
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1269427
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionAvian metapneumovirus (AMPV) belongs to Metapneumovirus genus of Paramyxoviridae family. Virus isolation, serology, and detection of genomic RNA are used as diagnostic methods for AMPV. The aim of the present study was to compare the detection of six subgroup A AMPV isolates (AMPV/A) viral RNA by using different conventional and real time RT-PCR methods. Two new RT-PCR tests and two real time RT-PCR tests, both detecting fusion (F) gene and nucleocapsid (N) gene were compared with an established test for the attachment (G) gene. All the RT-PCR tested assays were able to detect the AMPV/A. The lower detection limits were observed using the N-, F-based RRT-PCR and F-based conventional RT-PCR (10(0.3) to 10(1) TCID(50) mL(-1)). The present study suggests that the conventional F-based RT-PCR presented similar detection limit when compared to N-and F-based RRT-PCR and they can be successfully used for AMPV/A detection.
dc.description39
dc.description5
dc.description1445
dc.description1451
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description[03 14012-9]
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description[03 14012-9]
dc.languageen
dc.publisherUniv Federal Santa Maria
dc.publisherSanta Maria
dc.publisherBrasil
dc.relationCiencia Rural
dc.relationCienc. Rural
dc.rightsaberto
dc.sourceWeb of Science
dc.subjectavian metapneumovirus
dc.subjectG, F, N genes
dc.subjectreal time RT-PCR
dc.subjectRT-PCR
dc.subjectReverse Transcription-pcr
dc.subjectTurkey Rhinotracheitis
dc.subjectPneumovirus
dc.subjectVirus
dc.subjectSubgroups
dc.subjectAssay
dc.subjectGene
dc.titleComparative evaluation of conventional RT-PCR and real-time RT-PCR (RRT-PCR) for detection of avian metapneumovirus subtype A
dc.typeArtículos de revistas


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