Usefulness of concanavalin-A non-binding fraction of Strongyloides venezuelensis larvae to detect IgG and IgA in human strongyloidiasis

dc.creatorGonzaga, HT
dc.creatorRibeiro, VD
dc.creatorCunha, JP
dc.creatorUeta, MT
dc.creatorCosta-Cruz, JM
dc.date2011
dc.dateMAY
dc.date2014-07-30T19:07:29Z
dc.date2015-11-26T16:28:01Z
dc.date2014-07-30T19:07:29Z
dc.date2015-11-26T16:28:01Z
dc.date.accessioned2018-03-28T23:08:59Z
dc.date.available2018-03-28T23:08:59Z
dc.identifierDiagnostic Microbiology And Infectious Disease. Elsevier Science Inc, v. 70, n. 1, n. 78, n. 84, 2011.
dc.identifier0732-8893
dc.identifierWOS:000290290500012
dc.identifier10.1016/j.diagmicrobio.2011.01.016
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/73039
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/73039
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1269347
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionGlycosylated components from Strongyloides have an important role in parasite establishment and host recognition of these substances. Considering the sugar-binding capacity of lectins, such as concanavalin-A (Con-A), IgG and IgA detection in serum samples from strongyloidiasis patients was tested using different antigenic preparations. The total saline extract (SE) of Strongyloides venezuelensis filariform larvae was fractionated in Con-A column to obtain Con-A unbound (Con-A UF) and Con-A bound (Con-A BF) fractions. Sensitivity (Se), specificity (Sp), area under the ROC curve (AUC), likelihood ratio (LR), and correlation coefficients were calculated. Con-A UF showed the highest diagnostic parameters for IgG detection (Se 95.0%, Sp 92.5%, AUC 0.99, LR 12.7) and high correlation (r = 0.700) with SE. Con-A fractions did not clearly demonstrate any usefulness for IgA detection. In conclusion, the results obtained demonstrate that Con-A UF is an important source of specific peptides efficient to detect IgG in strongyloidiasis immunodiagnosis. (C) 2011 Elsevier Inc. All rights reserved.
dc.description70
dc.description1
dc.description78
dc.description84
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)
dc.descriptionFederal University of Uberlandia (UFU), Brazil
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageen
dc.publisherElsevier Science Inc
dc.publisherNew York
dc.publisherEUA
dc.relationDiagnostic Microbiology And Infectious Disease
dc.relationDiagn. Microbiol. Infect. Dis.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectStrongyloidiasis
dc.subjectStrongyloides stercoralis
dc.subjectGlycoproteins
dc.subjectSerodiagnosis
dc.subjectLinked-immunosorbent-assay
dc.subjectHuman Neurocysticercosis
dc.subjectStercoralis Infection
dc.subjectIntestinal Nematode
dc.subjectAntigenic Fractions
dc.subjectTaenia-saginata
dc.subjectDiagnosis
dc.subjectResponses
dc.subjectImmunodiagnosis
dc.subjectReactivity
dc.titleUsefulness of concanavalin-A non-binding fraction of Strongyloides venezuelensis larvae to detect IgG and IgA in human strongyloidiasis
dc.typeArtículos de revistas


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