Inhibition of avian metapneumovirus (AMPV) replication by RNA interference targeting nucleoprotein gene (N) in cultured cells

dc.creatorFerreira, HL
dc.creatorSpilki, FR
dc.creatorde Almeida, RS
dc.creatorSantos, MMAB
dc.creatorArns, CW
dc.date2007
dc.dateAPR
dc.date2014-11-18T14:04:39Z
dc.date2015-11-26T16:27:55Z
dc.date2014-11-18T14:04:39Z
dc.date2015-11-26T16:27:55Z
dc.date.accessioned2018-03-28T23:08:54Z
dc.date.available2018-03-28T23:08:54Z
dc.identifierAntiviral Research. Elsevier Science Bv, v. 74, n. 1, n. 77, n. 81, 2007.
dc.identifier0166-3542
dc.identifierWOS:000245614400011
dc.identifier10.1016/j.antiviral.2006.12.002
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/80565
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/80565
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/80565
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1269322
dc.descriptionAvian metapneumovirus (AMPV) is the primary causative agent of severe rhinotracheitis in turkeys. It is associated with swollen head syndrome in chickens and is the source of significant economic losses to animal food production. In this study, we designed specific short interfering RNA (siRNA) targeting the AMPV nucleoprotein (N) and fusion (F) genes. Three days post-virus infection, virus titration, real time RT-PCR, and RT-PCR assays were performed to verify the effect of siRNA in AMPV replication. A marked decrease in virus titers from transfected CER cells treated with siRNA/N was observed. Also, the production of N, F, and G mRNAs in AMPV was decreased. Results indicate that N-specific siRNA can inhibit virus replication. In future studies, a combination of siRNAs targeting the RNA polymerase complex may be used as a tool to study AMPV replication and/or antiviral therapy. (c) 2006 Elsevier B.V. All rights reserved.
dc.description74
dc.description1
dc.description77
dc.description81
dc.languageen
dc.publisherElsevier Science Bv
dc.publisherAmsterdam
dc.publisherHolanda
dc.relationAntiviral Research
dc.relationAntiviral Res.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectavian metapneumovirus
dc.subjectRNA interference
dc.subjectshort interfering RNA
dc.subjectinhibition
dc.subjectreal time PCR
dc.subjectInfluenza-virus Production
dc.subjectPolymerase Chain-reaction
dc.subjectTurkey Rhinotracheitis
dc.subjectAbsolute Quantification
dc.subjectPneumoviruses
dc.subjectSequence
dc.subjectSirna
dc.subjectTranscription
dc.subjectDegradation
dc.subjectInfection
dc.titleInhibition of avian metapneumovirus (AMPV) replication by RNA interference targeting nucleoprotein gene (N) in cultured cells
dc.typeArtículos de revistas


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